The RNAscope 2.5 High Definition (HD)—Red Assay, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH. The HD version is the most sensitive RNA ISH method and can be used for low expressing gene targets.
RNAscope™ 2.5 HD Assay - RED
The RNAscope 2.5 HD Reagent Kit-RED assay with it Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope.
The RNAscope 2.5 HD--RED Manual Assay Workflow Requires:
- RNAscope Target Probes (Catalog or Made-to-Order C1 Probes)
- RNAscope Control Probes (Select species-specific positive control probes and negative control probes)
- RNAscope Control Slides (optional)
- RNAscope 2.5 HD Reagent Kit—RED (Pretreatment Kit, Detection kit, and Wash Buffer included)
- Brightfield microscope or multispectral fluorescent imaging system
- HybEZ™ Hybridization System
