RNAscope™ VS Duplex Assay

RNAscope VS Mm- Slc17a1 and RNAscope VS Mm-Gad1 on Mouse Brain Tissue

RNAscope VS Mm- Slc17a1 and RNAscope VS Mm-Gad1 on Mouse Brain Tissue

RNAscope VS Mm-Grin1 and RNAscope VS Mm- Gad1 on Mouse Brain Tissue

RNAscope VS Mm-Grin1 and RNAscope VS Mm- Gad1 on Mouse Brain Tissue

The RNAscope VS Duplex Assay is designed as an automated assay on the DISCOVERY ULTRA Automated IHC/ISH slide staining systems from Roche. The RNAscope VS Duplex Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH.

RNAscope VS Duplex Kit is designed for simultaneous in situ detection of two RNA species. 

Common applications include co-localization studies to map co-expression of two targets within the same cellular context (e.g. secreted ligand and its receptor) or to profile gene expression in a specific cell type expressing a known marker (e.g. a specific stem cell marker). To distinguish between the two targets, individual probes are used in probe channels 1 (C1) and Channel 2 (C2). Channel 1 (C1)  is detected using HRP based reaction resulting in a dark brown, teal, or green signal (depending on the HRP based dye of choice) and Channel 2 (C2)  is detected using an AP Fast Red based reaction resulting in a red color. Due to the brightness of the red chromogen, it is advised that the lower expressed target be ordered in the C2 channel and stronger expressed target be ordered in the C1 channel.  The stained slides are visualized with a bright-field microscope.

The RNAscope VS Duplex assay may be utilized with any of the following HRP-based and AP-based detection kits from Roche Tissue Diagnostics. In addition to detection kits, other products listed below may also be required:


Available from Roche Tissue Diagnostics

ACD assay

Product name

Part number

Ordering code

RNAscope VS Duplex Reagent kit

mRNA Green Detection kit

mRNA RED Detection kit
mRNA DAB Detection kit
mRNA Teal Detection kit
mRNA Duplex AMP kit
mRNA Sample Prep kit
mRNA Link





Three mRNA ISH Duplex capabilities:

mRNA Green/RED





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