RNAscope Seminar - University of Cambridge - CAB, UK

Single-molecule RNA in situ hybridization for the detection of gene expression and alternative splicing in tissue

 

RNA in situ hybridization is a powerful technique for analyzing RNA expression at the single cell level within the morphological context, providing valuable spatial and cellular information missing from bulk tissue analysis.

The next-generation RNA ISH technologies called RNAscope® and BaseScope™ allow single RNA molecule detection in single cells and tissues with single-base resolution. 

Key features
 

  • Detection of a single RNA molecules. The RNAscope® probe design provides robustness against partial target RNA accessibility or degradation.
  • Signal amplification coupled with simultaneous background suppression strategy results in single-RNA-molecule detection even in partially degraded samples.
  • Provides cell-specific expression information in intact tissue architecture.
  • Works for virtually ANY gene from ANY species in ANY tissue.

Examples of applications of RNAscope® and BaseScope™ assay will be discussed, including

  • GPCRs and secreted molecules
  • Alternative splicing
  • Circular RNA
Location: University of Cambridge, Analytical Biotechnology (CABs) Cambridge, University West Site, Philippa Fawcett Dr, Cambridge, UK - Room: Lecture Hall
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