RNAscope® Technology Seminar - Oxford, UK

RNASCOPE® TECHNOLOGY 

June 9, 2017

12 - 1 pm

MRC Weatherall Institute of Molecular Medicine (WIMM)
University of Oxford
John Radcliffe Hospital
Oxford, UK


 

The RNAscope® technology is being routinely used for tissue-based gene expression analysis for target validation, pathobiology and biomarker development in every disease area. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS.

 

Matthew Nolan will present on the RNAscope® application in Neuroscience In-situ expression of post-mitotic transcription factors associated with projection neuron diversity in the adult post-mortem brain
 

 

In addition Farai Shah will give an overview on:

Visualizing gene expression and genetic variations in tissue environments of Neuroscience and Cancer related research by RNAscope® and BaseScope™

  • Detect mRNAs and lncRNAs at single molecule sensitivity by RNAscope® assay
  • Target specific splice junctions for aberrant alternative splicing in cancers by BaseScope™ assay
  • Characterize expression profiles of various checkpoint markers and therapeutic targets in the tumor microenvironment (TME)
  • Detection, characterization, and (co-)localization of mRNAs in the central and peripheral nervous system:
    • Co-expression with specific cell type markers
    • Sub-cellular localization of mRNA expression in cell body, axons or dendrites 
  • Distinguish specific homologues, paralogues, and orthologues by RNAscope® assay or BaseScope™ assay
  • Perform single cell analysis with morphological information in FFPE or frozen tissue
  • Pinpoint expression of novel RNAs of interest 
Location: MRC Weatherall Institute of Molecular Medicine (WIMM), University of Oxford, John Radcliffe Hospital, Oxford, UK

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