RNAscope® Technology Seminar - London, UK

Detection of RNA in the central and peripheral nervous system using the RNAscope® in situ hybridization assay

 

The RNAscope® technology is being routinely used for tissue-based gene expression analysis for neuroscience applications. In particular, the multiplexing capability for the simultaneous detection of multiple targets enables robust gene expression analysis and visualization of distinct cell populations within the nervous system. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex brain tissue, has advanced neuroscience research as seen in over 150 recent neuroscience publications.

 

This seminar will give an overview on:

Visualizing gene expression and genetic variations in Neuroscience research using RNAscope® and BaseScope™ Assays

  • Detect mRNAs and lncRNAs at single molecule sensitivity by RNAscope® assay
  • Target specific splice junctions for aberrant alternative splicing in cancers by BaseScope™ assay
  • Characterize expression profiles of various neuroscience markers in brain tissue
  • Detection, characterization, and (co-)localization of mRNAs in the central and peripheral nervous system:
    • Co-expression with specific cell type markers
    • Sub-cellular localization of mRNA expression in cell body, axons or dendrites 
  • Distinguish specific homologues, paralogues, and orthologues by RNAscope® assay or BaseScope™ assay
  • Perform single cell analysis with morphological information in FFPE  or fresh frozen tissue
  • Pinpoint expression of novel RNAs of interest 
Location: Seminar Room, Burlington Danes Building, Hammersmith Campus, Imperial College of London

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