RNASCOPE^® TECHNOLOGY 

Tuesday March 28, 2017

1:00 - 2:00

UCL - Institute of Ophthalmology
11-43 Bath Street, London

Seminar Room

The RNAscope™ technology is being routinely used for tissue-based gene expression analysis for target validation, pathobiology and biomarker development in every disease area. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS.

This seminar will give an overview on RNAscope™ and BaseScope™ assays, the two highly specific and sensitive ISH methods that allow visualizing gene expression and genetic variations in tissue environments:

• Detection, characterization, and (co-)localization of mRNAs in the central and peripheral nervous system: 
  • Co-expression with specific cell type markers
  • Sub-cellular localization of mRNA expression in cell body, axons or dendrites
• Validation of target mRNA expression after high-throughput transcriptome analysis
• Detection of mRNA in the nervous system when no (reliable) antibodies are available, including GPCRs and long non-coding RNA (lncRNA)
• Validation of (cell type-specific) genetic modifications including knock-out models or transgene expression
• Characterization of specific alternatively spliced variants in single cells
• Visualizing expression of circular RNAs at subcellular level in tissue environment