RNAscope

Inflammation is strongly associated with pancreatic ductal adenocarcinoma (PDAC), a highly lethal malignancy. Dysregulated RNA splicing factors have been widely reported in tumorigenesis, but their involvement in pancreatitis and PDAC is not well understood. Here, we report that the splicing factor SRSF1 is highly expressed in pancreatitis, PDAC precursor lesions, and tumors. Increased SRSF1 is sufficient to induce pancreatitis and accelerate KRASG12D-mediated PDAC.

The evolution of new traits enables expansion into new ecological and behavioural niches. Nonetheless, demonstrated connections between divergence in protein structure, function and lineage-specific behaviours remain rare. Here we show that both octopus and squid use cephalopod-specific chemotactile receptors (CRs) to sense their respective marine environments, but structural adaptations in these receptors support the sensation of specific molecules suited to distinct physiological roles.

Erythropoietin (Epo) is the master regulator of erythropoiesis and oxygen homeostasis. Despite its physiological importance, the molecular and genomic contexts of the cells responsible for renal Epo production remain unclear, limiting more-effective therapies for anemia. Here, we performed single-cell RNA and transposase-accessible chromatin (ATAC) sequencing of an Epo reporter mouse to molecularly identify Epo-producing cells under hypoxic conditions.

High-quality mouse dorsal root ganglion (DRG) cryostat sections are crucial for proper immunochemistry staining and RNAscope studies in the research of inflammatory and neuropathic pain, itch, as well as other peripheral neurological conditions. However, it remains a challenge to consistently obtain high-quality, intact, and flat cryostat sections onto glass slides because of the tiny sample size of the DRG tissue. So far, there is no article describing an optimal protocol for DRG cryosectioning.

Related the profile of some toxicological aspect of some COVID -19 vaccine and in specifc way to the rare effect like pericarditis or trombosys are reported in this work some relevant literature involved in the effect played by the SPIKE PROTEINS and its link on epithelial tissue ACE receptor, the Graphene (if present) and under some magnetic field or electrical condition. An experimental hypotesys is submitted to the reseacher in order to produce a global conclusion of toxicological interest.

特定のタンパク質や遺伝子発現の組織内局在・細胞 内局在を明らかにする組織学的手法は，生命科学・医 学における生体機能解析の基幹手法である．代表的な 組織学的手法の一つである in situ hybridization 法 （ISH）は，DNA・RNA 等核酸を標的にするという特 性から，遺伝子改変動物を用いた機能解析，AAV ウ イルスによる局所的機能調節など，近年大きく発展を 続けている分子生物学的な手法との併用に強みをもつ 手法である．特に神経系の研究分野では，近年のsinglecell RNA シーケンス解析により，単一神経核/領域内 で遺伝子発現プロファイルの異なる多数の細胞集団が 混在していることが明らかになりつつあり1,2，細胞レ ベルの分解能で mRNA 局在解析を行うことができる ISH はより重要性を増している3 ．また，より短いプ ローブ長での高感度化や手法の簡便化などを目標に手 法の改良が続いており，従来の感度では検出できな かった低発現量遺伝子の解析など，より応用の幅が広 がっている．本稿では，タンパク質を検出標的とする 免疫染色法と比較した ISH の特徴，近年の ISH 高感度 化の試みに加え，神経科学分野の未解明領域への応用 について概説する．

In regenerative species, retinal injury induces Müller glia to de-differentiate and proliferate to replace lost cells, whereas in mammals, an initial repair response is rapidly aborted. We found that _Plagl1_, a maternally-imprinted transcription factor gene that is dynamically expressed post-insult, sustains murine glial quiescence at early postnatal stages. _Plagl1_+/-pat null mutant Müller glia translocate, proliferate and differentiate into inner-retinal neurons and photoreceptors.

Pulmonary fibrosis is an interstitial lung disease caused by various factors such as exposure to workplace environmental contaminants, drugs, or X-rays. Epithelial cells are among the driving factors of pulmonary fibrosis. Immunoglobulin A (IgA), traditionally thought to be secreted by B cells, is an important immune factor involved in COVID-19 infection and vaccination. In current study, we found lung epithelial cells were involved in IgA secretion which, in turn, promoted pulmonary fibrosis.

Food cues elicit the body’s responses and subsequent food consumption. The magnitude of the response to food cues is a crucial risk factor for obesity. However, the underlying neural mechanism of how the cues of edible food promote feeding remains unclear. Here we demonstrated that the peptidergic CRH neurons in the lateral hypothalamic area are the missing link that connects food cues to food consumption. We first established the activation of those neurons triggered by food cues with multiple assays.