RNAscope™ assay is an innovative and proprietary RNA in situ hybridization (ISH) assay based on ACD’s patented technology with signal amplification and simultaneous background noise suppression. RNAscope™ assay delivers quantitative, sensitive and specific molecular detection of RNA species on a cell-by-cell basis with anatomical context in a single assay.
Download the Technote:
Tech Note for using ImageJ to analyze RNAscope™ and BaseScope™ data
- Pathologists who may or may not be familiar with RNAscope™ ISH
- New and existing RNAscope™ Assay users (manual and automated assay platforms)
- Traditional ISH users new to the RNAscope™ technology
- IHC users interested in RNA ISH for complementary studies
In this webinar, we will focus on how to review and analyze RNAscope™ assay data images successfully. We will cover the following topics:
• Assess examples of RNAscope™ images with poor, medium and high-quality target RNA staining
• Learn best practices for accurately interpreting your RNAscope™ images and discuss RNAscope™ scoring tips
• Review available types of data analysis and data presentation using HALOTM and open-source software on RNAscope™ images
• Learn how to best capture high quality images for subsequent data analysis
• Review the current workflow of quantitative data analysis by the Pharma Assay Services Team
