Harnessing in situ transcriptomics to reveal complex tissue architecture at single-cell resolution
Omer Bayraktar, Ph.D.
Wellcome Sanger Institute
Xiao-Jun Ma, Ph.D.
Chief Scientific Officer, Advanced Cell Diagnostics
Outline:

Sequencing of RNA (RNASeq) can provide a trove of useful information for quantification and characterization of the transcriptome. During bulk sequencing, however, spatial and cellular heterogeneity information is lost. Enter single-cell–level analysis, which can provide unprecedented detail on cellular diversity in development, health, and disease. This emerging area of spatial transcriptomics aims to map a single-cell–level molecular atlas of human tissues in situ. However, mapping entire tissue areas at single-cell quantitative resolution presents significant challenges for imaging and data analysis. In this webinar, efforts to develop large-scale in situ transcriptomics will be described, using the mouse brain as a model system for mapping the molecular diversity of neuronal and glial cell types. The experimental workflow includes automated tissue processing, imaging and analysis for large-scale multiplex mapping, and quantification of gene expression across brain regions in situ at single-cell resolution. The technology that makes this high-resolution mapping possible will be described and compared with other current methods.

 

Watch the on-demand recording of this webinar here

Learning Objectives:

During the webinar, the speakers will:

  • Describe the power and usefulness of in situ transcriptomics
  • Discuss the application of this technology to large-scale tissue mapping in the mouse brain
  • Explain how in situ transcriptomics (using RNAScope technology) can complement RNASeq and single-cell RNASeq
  • Answer audience questions during the live broadcast!

To learn more about products or technologies related to this webinar, go to http://rna.acdbio.com/transcriptomics.

This webinar will last for approximately 60 minutes.

X
Contact Us

Complete one of the two forms below and we will get back to you.

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Bio-Techne

19 Barton Lane  
Abingdon Science Park
Abingdon
OX14 3NB
United Kingdom
Phone 2: +44 1235 529449
Fax: +44 1235 533420

 

 

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china.ACD@bio-techne.com
For general information: Leon.Li@bio-techne.com
Web: www.acdbio.com/cn

For general information: Info.ACD@bio-techne.com
For place an order: order.ACD@bio-techne.com
For product support: support.ACD@bio-techne.com
For career opportunities: hr.ACD@bio-techne.com