ACD has partnered with Indica Labs to offer quantitative RNAscope image analysis. This advanced digital pathology image analysis solution brings objective and accurate quantification to RNA in situ hybridization, enabling a new generation of applications based on single-cell analysis. With HALO, gene expression can be measured quantitatively at single-cell resolution and interpreted by pathologists within the histopathological context.
Halo Software by Indica Labs
Sample Images
The benefits of image analysis using HALO software after RNAscope staining include:
- quantitative and reproducible RNAscope spot counting
- single-plex and multiplex ISH quantification
- brightfield and fluorescent ISH quantification
- cell-by-cell expression profiles
- high throughput computing power for faster turnaround time
- whole-slide image or field-of-view image analysis
- manual and automatic selection of distinct cell populations
HALO image analysis is compatible with all RNAscope Manual and Automated Assays:
- RNAscope® 2.0 HD Reagent Kit—BROWN
- RNAscope® 2.0 HD Reagent Kit—RED
- RNAscope® 2-Plex Reagent Kit
- RNAscope® Multiplex Fluorescent Reagent Kit
- RNAscope® LS Reagent Kit—BROWN
- RNAscope® LS Reagent Kit—RED
- RNAscope® VS Reagent Kit—BROWN
- RNAscope® VS Reagent Kit—RED
ACD offers image analysis as part of our Pharma Assay Services (Learn more)
The HALO image analysis software is also available for purchase and deployment within your organization. For more information contact info@indicalab.com or visit Indicalab.com.
