Probes for INS
ACD can configure probes for the various manual and automated assays for INS for RNAscope Assay, or for Basescope Assay compatible for your species of interest.
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J Comp Neurol.
2019 May 09
Levy J, Facchinetti P, Jan C, Achour M, Bouvier C, Brunet JF, Delzescaux T, Giuliano F.
PMID: 31071232 | DOI: 10.1002/cne.24713
From a whole macaque (m. fascicularis) brain, serial transversal sections of the brainstem were made using a blockface setup that takes high-definition pictures of the organ surface, before microtome slicing. Different histological techniques were applied to series of sections which allowed us to identify RTN neurons among Phox2b immunoreactive cells within the macaque brainstem. Our findings rely on the specific signature previously assessed in rats for chemosensitive neurons of the parafacial region: Phox2b/VGluT2/Galanin. Blockface photographs allowed us to correct histology-induced deformations on digitized sections. Series of sections were virtually stacked and ppGal/Phox2b positive cells identified to provide with the first 3D description of the main chemosensitive brainstem region in non-human primates.
| Description | ||
|---|---|---|
| sense Example: Hs-LAG3-sense | Standard probes for RNA detection are in antisense. Sense probe is reverse complent to the corresponding antisense probe. | |
| Intron# Example: Mm-Htt-intron2 | Probe targets the indicated intron in the target gene, commonly used for pre-mRNA detection | |
| Pool/Pan Example: Hs-CD3-pool (Hs-CD3D, Hs-CD3E, Hs-CD3G) | A mixture of multiple probe sets targeting multiple genes or transcripts | |
| No-XSp Example: Hs-PDGFB-No-XMm | Does not cross detect with the species (Sp) | |
| XSp Example: Rn-Pde9a-XMm | designed to cross detect with the species (Sp) | |
| O# Example: Mm-Islr-O1 | Alternative design targeting different regions of the same transcript or isoforms | |
| CDS Example: Hs-SLC31A-CDS | Probe targets the protein-coding sequence only | |
| EnEm | Probe targets exons n and m | |
| En-Em | Probe targets region from exon n to exon m | |
| Retired Nomenclature | ||
| tvn Example: Hs-LEPR-tv1 | Designed to target transcript variant n | |
| ORF Example: Hs-ACVRL1-ORF | Probe targets open reading frame | |
| UTR Example: Hs-HTT-UTR-C3 | Probe targets the untranslated region (non-protein-coding region) only | |
| 5UTR Example: Hs-GNRHR-5UTR | Probe targets the 5' untranslated region only | |
| 3UTR Example: Rn-Npy1r-3UTR | Probe targets the 3' untranslated region only | |
| Pan Example: Pool | A mixture of multiple probe sets targeting multiple genes or transcripts | |
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