Application of CRISPR-Based Technology in Medical Research and Disease Treatment

CRISPR

2022 Jan 01

Zhang, Z;Wang, P;Liu, J;
| DOI: 10.1007/978-981-16-8504-0_4

Seeking treatments for human diseases is an important goal and motivation of biomedical research. Over thousands of years, humans have gradually formed a series of treatment plans for diseases, which have gradually evolved into modern medicine. Although many common diseases have been overcome, a cure for diseases caused by genetic mutations, including congenital genetic diseases, is still lacking. To overcome genetic diseases caused by DNA mutations, new strategies must be developed. An example that has received much attention is gene therapy, which correct or compensate diseases caused by defects and abnormal genes by introducing foreign normal genes into target cells to achieve the purpose of treatment. Although these methods have been proven effective and are undergoing clinical trials, the ideal treatment is to fundamentally correct the mutated DNA.

Telomere dysfunction in ageing and age-related diseases

Nature cell biology

2022 Feb 01

Rossiello, F;Jurk, D;Passos, JF;d'Adda di Fagagna, F;
PMID: 35165420 | DOI: 10.1038/s41556-022-00842-x

Ageing organisms accumulate senescent cells that are thought to contribute to body dysfunction. Telomere shortening and damage are recognized causes of cellular senescence and ageing. Several human conditions associated with normal ageing are precipitated by accelerated telomere dysfunction. Here, we systematize a large body of evidence and propose a coherent perspective to recognize the broad contribution of telomeric dysfunction to human pathologies.

Identification and three-dimensional reconstruction of oxytocin receptor expressing astrocytes in the rat and mouse brain

STAR protocols

2022 Mar 18

Althammer, F;Krause, EG;de Kloet, AD;Smith, J;Grinevich, V;Charlet, A;Stern, JE;
PMID: 35199030 | DOI: 10.1016/j.xpro.2022.101160

Here, we present a step-by-step protocol for three-dimensional reconstruction of astrocyte morphology, applied to the central amygdala oxytocin receptor-expressing astrocytes. This includes RNAse-free perfusion, combination of RNAscope and immunohistochemistry, and confocal imaging. This protocol provides detailed information about tissue handling and a comprehensive description of the RNAScope technique to label rat and mouse oxytocin receptor mRNA. We also describe three-dimensional reconstruction that allows the assessment of more than 70 different cellular parameters, powerful for studying astrocyte morphology and astrocyte-astrocyte interactions. For complete details on the use and execution of this protocol, please refer to Wahis et al. (2021) and Althammer et al. (2020).

Investigating an Increased Uterine Cancer Risk in Breast Cancer Patients

Oncology Times

2022 Feb 05

Froelich, W;
| DOI: 10.1097/01.cot.0000820704.81927.64

Studies show that postmenopausal women with breast cancer treated with long-term use of tamoxifen are at an increased risk for uterine cancer, but why this occurs has been somewhat of a mystery.

Lifelong single-cell profiling of cranial neural crest diversification in zebrafish

Nature communications

2022 Jan 10

Fabian, P;Tseng, KC;Thiruppathy, M;Arata, C;Chen, HJ;Smeeton, J;Nelson, N;Crump, JG;
PMID: 35013168 | DOI: 10.1038/s41467-021-27594-w

The cranial neural crest generates a huge diversity of derivatives, including the bulk of connective and skeletal tissues of the vertebrate head. How neural crest cells acquire such extraordinary lineage potential remains unresolved. By integrating single-cell transcriptome and chromatin accessibility profiles of cranial neural crest-derived cells across the zebrafish lifetime, we observe progressive and region-specific establishment of enhancer accessibility for distinct fates. Neural crest-derived cells rapidly diversify into specialized progenitors, including multipotent skeletal progenitors, stromal cells with a regenerative signature, fibroblasts with a unique metabolic signature linked to skeletal integrity, and gill-specific progenitors generating cell types for respiration. By retrogradely mapping the emergence of lineage-specific chromatin accessibility, we identify a wealth of candidate lineage-priming factors, including a Gata3 regulatory circuit for respiratory cell fates. Rather than multilineage potential being established during cranial neural crest specification, our findings support progressive and region-specific chromatin remodeling underlying acquisition of diverse potential.

Psilocybin targets a common molecular mechanism for cognitive impairment and increased craving in alcoholism

Science advances

2021 Nov 19

Meinhardt, MW;Pfarr, S;Fouquet, G;Rohleder, C;Meinhardt, ML;Barroso-Flores, J;Hoffmann, R;Jeanblanc, J;Paul, E;Wagner, K;Hansson, AC;Köhr, G;Meier, N;von Bohlen Und Halbach, O;Bell, RL;Endepols, H;Neumaier, B;Schönig, K;Bartsch, D;Naassila, M;Spanagel, R;Sommer, WH;
PMID: 34788104 | DOI: 10.1126/sciadv.abh2399

[Figure: see text].

Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas

Science advances

2021 Nov 19

Heino, S;Fang, S;Lähde, M;Högström, J;Nassiri, S;Campbell, A;Flanagan, D;Raven, A;Hodder, M;Nasreddin, N;Xue, HH;Delorenzi, M;Leedham, S;Petrova, TV;Sansom, O;Alitalo, K;
PMID: 34788095 | DOI: 10.1126/sciadv.abj0512

[Figure: see text].

REV-ERB nuclear receptors in the suprachiasmatic nucleus control circadian period and restrict diet-induced obesity

Science advances

2021 Oct 29

Adlanmerini, M;Krusen, BM;Nguyen, HCB;Teng, CW;Woodie, LN;Tackenberg, MC;Geisler, CE;Gaisinsky, J;Peed, LC;Carpenter, BJ;Hayes, MR;Lazar, MA;
PMID: 34705514 | DOI: 10.1126/sciadv.abh2007

[Figure: see text].

The CD22-IGF2R interaction is a therapeutic target for microglial lysosome dysfunction in Niemann-Pick type C

Science translational medicine

2021 Dec 01

Pluvinage, JV;Sun, J;Claes, C;Flynn, RA;Haney, MS;Iram, T;Meng, X;Lindemann, R;Riley, NM;Danhash, E;Chadarevian, JP;Tapp, E;Gate, D;Kondapavulur, S;Cobos, I;Chetty, S;Pașca, AM;Pașca, SP;Berry-Kravis, E;Bertozzi, CR;Blurton-Jones, M;Wyss-Coray, T;
PMID: 34851695 | DOI: 10.1126/scitranslmed.abg2919

[Figure: see text].

Molecular self-avoidance in synaptic neurexin complexes

Science advances

2021 Dec 17

Wang, CY;Trotter, JH;Liakath-Ali, K;Lee, SJ;Liu, X;Südhof, TC;
PMID: 34919427 | DOI: 10.1126/sciadv.abk1924

[Figure: see text].

Abstract LBA020: Targeting FGFR2c isoform, a novel therapeutic target with FGFR inhibitor in endometrial cancer

Poster Presentations - Late-Breaking Proffered Abstracts

2021 Dec 01

Sengal, A;Bonazzi, V;Kondrashova, O;Perrin, L;Chetty, N;Smith, D;Gil-Moreno, A;Colas, E;Pollock, P;
| DOI: 10.1158/1535-7163.targ-21-lba020

PURPOSE: Endometrial cancer (EC) is the most frequently diagnosed gynaecological cancer. The majority of women with EC are treated surgically and have a good outcome, however 25-30% of patients presenting with metastases or recurrent disease do not have effective therapies and have

Neural control of affiliative touch in prosocial interaction

Nature

2021 Oct 13

Wu, YE;Dang, J;Kingsbury, L;Zhang, M;Sun, F;Hu, RK;Hong, W;
PMID: 10.1038/s41586-021-03962-w | DOI: Ethics declarations

Pages

	Description
sense Example: Hs-LAG3-sense	Standard probes for RNA detection are in antisense. Sense probe is reverse complent to the corresponding antisense probe.
Intron# Example: Mm-Htt-intron2	Probe targets the indicated intron in the target gene, commonly used for pre-mRNA detection
Pool/Pan Example: Hs-CD3-pool (Hs-CD3D, Hs-CD3E, Hs-CD3G)	A mixture of multiple probe sets targeting multiple genes or transcripts
No-XSp Example: Hs-PDGFB-No-XMm	Does not cross detect with the species (Sp)
XSp Example: Rn-Pde9a-XMm	designed to cross detect with the species (Sp)
O# Example: Mm-Islr-O1	Alternative design targeting different regions of the same transcript or isoforms
CDS Example: Hs-SLC31A-CDS	Probe targets the protein-coding sequence only
EnEm	Probe targets exons n and m
En-Em	Probe targets region from exon n to exon m
Retired Nomenclature
tvn Example: Hs-LEPR-tv1	Designed to target transcript variant n
ORF Example: Hs-ACVRL1-ORF	Probe targets open reading frame
UTR Example: Hs-HTT-UTR-C3	Probe targets the untranslated region (non-protein-coding region) only
5UTR Example: Hs-GNRHR-5UTR	Probe targets the 5' untranslated region only
3UTR Example: Rn-Npy1r-3UTR	Probe targets the 3' untranslated region only
Pan Example: Pool	A mixture of multiple probe sets targeting multiple genes or transcripts

Search form

Probes for INS

Content for comparison

Species

Gene

Platform

Channel

HiPlex Channel

Product

Research area

Product sub type

Sample Compatibility

Category

Application

Pages

Advanced Cell Diagnostics

Bio-Techne

Advanced Cell Diagnostics China