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Search

Probes for HIV-1

ACD can configure probes for the various manual and automated assays for HIV-1 for RNAscope Assay, or for Basescope Assay compatible for your species of interest.

  • Probes for HIV-1 (0)
  • Kits & Accessories (0)
  • Support & Documents (0)
  • Publications (3)
  • Image gallery (0)
Refine Probe List

Content for comparison

Gene

  • HIV-1 (19) Apply HIV-1 filter
  • TBD (16) Apply TBD filter
  • HIV (7) Apply HIV filter
  • HIV1 (3) Apply HIV1 filter
  • (-) Remove SIV filter SIV (2)
  • GAPDH (1) Apply GAPDH filter
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  • SIVMM32H (1) Apply SIVMM32H filter
  • IL-1β (1) Apply IL-1β filter
  • Slc12a3 (1) Apply Slc12a3 filter
  • SIVDeltaB670 (1) Apply SIVDeltaB670 filter
  • V-HIV Clade B anti-sense (1) Apply V-HIV Clade B anti-sense filter
  • CD30 (1) Apply CD30 filter
  • vpr (1) Apply vpr filter
  • HIV-1 clade B (1) Apply HIV-1 clade B filter
  • HIV-1  (1) Apply HIV-1  filter
  • subtype C HIV-1 (1) Apply subtype C HIV-1 filter
  • influenza PB1 (1) Apply influenza PB1 filter
  • HIV-1 gag-pol (1) Apply HIV-1 gag-pol filter
  • V-HIV1-CladeB (1) Apply V-HIV1-CladeB filter
  • SHIVBG505 (gag-pol (1) Apply SHIVBG505 (gag-pol filter
  • vif-vpu-nef) (1) Apply vif-vpu-nef) filter
  • HIV-1 clade B anti-sense (1) Apply HIV-1 clade B anti-sense filter
  • (-) Remove HIV-1 gag/pol filter HIV-1 gag/pol (1)
  • HIV-1 gag (1) Apply HIV-1 gag filter
  • HERV-Kenv-C1 (1) Apply HERV-Kenv-C1 filter
  • HIV-1 gag-pol-C2 (1) Apply HIV-1 gag-pol-C2 filter

Product

  • RNAscope (1) Apply RNAscope filter
  • RNAscope 2.5 HD Red assay (1) Apply RNAscope 2.5 HD Red assay filter

Research area

  • Infectious Disease (2) Apply Infectious Disease filter
  • HIV (1) Apply HIV filter
  • Inflammation (1) Apply Inflammation filter

Category

  • (-) Remove Publications filter Publications (3)
CRISPR/Cas9-Induced Mutagenesis Corroborates the Role of Transportin-SR2 in HIV-1 Nuclear Import

Microbiology spectrum

2021 Oct 31

Janssens, J;Blokken, J;Lampi, Y;De Wit, F;Zurnic Bonisch, I;Nombela, I;Van de Velde, P;Van Remoortel, B;Gijsbers, R;Christ, F;Debyser, Z;
PMID: 34612665 | DOI: 10.1128/Spectrum.01336-21

To infect nondividing cells, HIV-1 needs to cross the nuclear membrane. The importin transportin-SR2 (TRN-SR2 or transportin-3) has been proposed to mediate HIV-1 nuclear import, but the detailed mechanism remains unresolved. The direct interaction of TRN-SR2 with HIV-1 integrase (IN) has been proposed to drive HIV-1 nuclear import. Alternatively, TRN-SR2 may play an indirect role by mediating nuclear import of cleavage and polyadenylation specificity factor 6 (CPSF6). To unravel the role of TRN-SR2, we designed CRISPR/Cas9 guide RNAs targeting different exons of TNPO3. Although this approach failed to generate full knockouts, monoallelic knockout clones were generated with indel mutations. HIV-1 replication was hampered in those clones at the level of HIV-1 nuclear import without an effect on the cellular distribution of the TRN-SR2 cargoes CPSF6 or alternative splicing factor1/pre-mRNA splicing factor SF2 (ASF/SF2). Recombinant ΔV105 TRN-SR2 expressed in clone 15.15 was 2-fold impaired for interaction with HIV-1 IN and classified as an interaction mutant. Our data support a model whereby TRN-SR2 acts as a cofactor of HIV-1 nuclear import without compromising the nuclear import of cellular cargoes. CRISPR/Cas9-induced mutagenesis can be used as a method to generate interface mutants to characterize host factors of human pathogens. IMPORTANCE Combination antiretroviral therapy (cART) effectively controls HIV-1 by reducing viral loads, but it does not cure the infection. Lifelong treatment with cART is a prerequisite for sustained viral suppression. The rapid emergence of drug-resistant viral strains drives the necessity to discover new therapeutic targets. The nuclear import of HIV-1 is crucial in the HIV-1 replication cycle, but the detailed mechanism remains incompletely understood. This study provides evidence that TRN-SR2 directly mediates HIV-1 nuclear import via the interaction with HIV-1 integrase. The interaction between those proteins is therefore a promising target toward a rational drug design which could lead to new therapeutic strategies due to the bottleneck nature of HIV-1 nuclear import.
Imaging lymphoid tissues in nonhuman primates to understand SIV pathogenesis and persistence.

Curr Opin Virol.

2016 Aug 01

Deleage C, Turkbey B, Estes JD.
PMID: 27490446 | DOI: 10.1016/j.coviro.2016.07.002

CD4+ T cells are the primary HIV-1 target cell, with the vast majority of these cells residing within lymphoid tissue compartments throughout the body. Predictably, HIV-1 infection, replication, localization, reservoir establishment and persistence, as well as associated host immune and inflammatory responses and disease pathology principally take place within the tissues of the immune system. By virture of the fact that the virus-host struggle is played out within lymphoid and additional tissues compartments in HIV-1 infected individuals it is critical to understand HIV-1 infection and disease within these relevant tissue sites; however, there are obvious limitations to studying these dynamic processes in humans. Nonhuman primate (NHP) research has provided a vital bridge between basic and preclinical research and clinical studies, with experimental SIV infection of NHP models offering unique opportunities to understand key processes of HIV-1 infection and disease that are either not practically feasible or ethical in HIV-1 infected humans. In this review we will discuss current approaches to studying the tissue based immunopathogenesis of AIDS virus infection in NHPs, including both analyses of tissues obtained at biopsy or necropsy and complementary non-invasive imaging approaches that may have practical utility in monitoring HIV-1 disease in the clinical setting.

CTLA-4+PD-1− Memory CD4+ T Cells Critically Contribute to Viral Persistence in Antiretroviral Therapy-Suppressed, SIV-Infected Rhesus Macaques

Immunity.

2017 Oct 17

McGary CS, Deleage C, Harper J, Micci L, Ribeiro SP, Paganini S, Kuri-Cervantes L, Benne C, Ryan ES, Balderas R, Jean S, Easley K, Marconi V, Silvestri G, Estes JD, Sekaly RP, Paiardini M.
PMID: 29045906 | DOI: 10.1016/j.immuni.2017.09.018

Antiretroviral therapy (ART) suppresses viral replication in HIV-infected individuals but does not eliminate the reservoir of latently infected cells. Recent work identified PD-1+ follicular helper T (Tfh) cells as an important cellular compartment for viral persistence. Here, using ART-treated, SIV-infected rhesus macaques, we show that CTLA-4+PD-1- memory CD4+ T cells, which share phenotypic markers with regulatory T cells, were enriched in SIV DNA in blood, lymph nodes (LN), spleen, and gut, and contained replication-competent and infectious virus. In contrast to PD-1+ Tfh cells, SIV-enriched CTLA-4+PD-1- CD4+ T cells were found outside the B cell follicle of the LN, predicted the size of the persistent viral reservoir during ART, and significantly increased their contribution to the SIV reservoir with prolonged ART-mediated viral suppression. We have shown that CTLA-4+PD-1- memory CD4+ T cells are a previously unrecognized component of the SIV and HIV reservoir that should be therapeutically targeted for a functional HIV-1 cure.

X
Description
sense
Example: Hs-LAG3-sense
Standard probes for RNA detection are in antisense. Sense probe is reverse complent to the corresponding antisense probe.
Intron#
Example: Mm-Htt-intron2
Probe targets the indicated intron in the target gene, commonly used for pre-mRNA detection
Pool/Pan
Example: Hs-CD3-pool (Hs-CD3D, Hs-CD3E, Hs-CD3G)
A mixture of multiple probe sets targeting multiple genes or transcripts
No-XSp
Example: Hs-PDGFB-No-XMm
Does not cross detect with the species (Sp)
XSp
Example: Rn-Pde9a-XMm
designed to cross detect with the species (Sp)
O#
Example: Mm-Islr-O1
Alternative design targeting different regions of the same transcript or isoforms
CDS
Example: Hs-SLC31A-CDS
Probe targets the protein-coding sequence only
EnEmProbe targets exons n and m
En-EmProbe targets region from exon n to exon m
Retired Nomenclature
tvn
Example: Hs-LEPR-tv1
Designed to target transcript variant n
ORF
Example: Hs-ACVRL1-ORF
Probe targets open reading frame
UTR
Example: Hs-HTT-UTR-C3
Probe targets the untranslated region (non-protein-coding region) only
5UTR
Example: Hs-GNRHR-5UTR
Probe targets the 5' untranslated region only
3UTR
Example: Rn-Npy1r-3UTR
Probe targets the 3' untranslated region only
Pan
Example: Pool
A mixture of multiple probe sets targeting multiple genes or transcripts

Enabling research, drug development (CDx) and diagnostics

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