Contact Us / Request a Quote Download Manuals
Advanced Cell Diagnostics Advanced Cell Diagnostics

Search form

Please sign in
  • Log In
  • Register
  • How to Order
  • What to Buy
0 My Cart
X

You have no items in your shopping cart.

Menu
X
  • Products +
    RNAscope™/BaseScope™/ miRNAscope™
    +
    • Assay Selection Guide
    Target Probes
    +
    • All About Probes
    • Catalog Probes
    • Probe Sets
    • New Probe Request
    Manual Assays
    +
    RNAscope™ Chromogenic
    • Overview
    • RNAscope™ 2.5 HD Assay-Brown
    • RNAscope™ 2.5 HD Assay-Red
    • RNAscope™ 2.5 HD Duplex Assay
    RNAscope™ Multiplex Fluorescent
    • Overview
    • RNAscope™ HiPlex v2 Assay
    • RNAscope™ Multiplex Fluorescent V2
    BaseScope™
    • Overview
    • BaseScope™ Assay Red
    • BaseScope™ Duplex Assay
    miRNAscope™
    • Overview
    • miRNAscope™ Assay red
    • RNAscope™ Plus smRNA-RNA Assay
    DNAscope™
    • Overview
    • DNAscope™ Duplex Assay
    Automated Assays
    +
    For Lunaphore COMET™
    • RNAscope™ HiPlex Pro for COMET™
    For Leica systems
    • Overview
    • RNAscope™ 2.5 LS Assay-Brown
    • RNAscope™ 2.5 LS Assay-Red
    • RNAscope™ 2.5 LS Duplex Assay
    • RNAscope™ Multiomic LS Assay
    • RNAscope™ 2.5 LS Fluorescent Multiplex Assay
    • RNAscope™ 2.5 LSx Reagent Kit-BROWN
    • RNAscope™ 2.5 LSx Reagent Kit-RED
    • BaseScope™ LS Reagent Kit – RED
    • miRNAscope LS Reagent Kit Red
    • RNAscope™ Plus smRNA-RNA LS Assay
    Roche DISCOVERY ULTRA system
    • Overview
    • RNAscope™ VS Universal HRP
    • RNAscope™ VS Universal AP
    • RNAscope™ VS Duplex Assay
    • BaseScope™ VS Reagent Kit – RED
    RNA-Protein Co-Detection Assay
    +
    • RNAscope HiPlex-IMC™ Co-Detection
    • Integrated Codetection Assay
    • Sequential RNA Protein Detection
    Software
    +
    • Overview
    • Aperio RNA ISH Algorithm
    • HALO® image analysis platform
    Controls & Accessories
    +
    • RNAscope™
    • BaseScope™
    • miRNAscope™
    • Accessories
    How to Order
    +
    • Ordering Instructions
    • What to Buy
  • Services +
    Professional Assay Services
    +
    • Our Services
    • Multiomic Services
    • Biomarker Assay Development
    • Cell & Gene Therapy Services
    • Clinical Assay Development
    • Tissue Bank & Sample Procurement
    • Image Analysis
    Benefits
    +
    • Your Benefits
    • Certified Providers
    How to Order
    +
    • Ordering Process
    • Contact Services
  • Areas of Research +
    Most Popular
    +
    • COVID-19 Coronavirus
    • Single Cell Analysis
    • Whole-Mount
    • Anatomic Pathology Panels
    • Neuroscience
    • Inflammation
    • Gene Therapy/AAV
    • Stem Cell
    • Immuno-oncology
    • Liver Research
    • Cardiovascular & Skeletal Muscle Research
    Cell & Gene Therapy
    +
    • Gene Therapy
    • Gene Therapy/AAV
    • siRNA/ASO
    • Cell Therapy
    Cancer
    +
    • Breast Cancer
    • EGFRvIII Splice Variant
    • HPV Related Cancer
    • Immuno-oncology
    • Lung Cancer
    • PDx
    • Prostate Cancer
    • Point Mutation
    • CDR3 for TCR
    Viral
    +
    • COVID-19 Coronavirus
    • HIV & SIV
    • Infectious Disease
    • Zika Virus
    Pathways
    +
    • AKT
    • JAK STAT
    • WNT B-Catenin
    Neuroscience
    +
    Neuroscience
    • Neural Development
    • Neuronal Cell Types
    • Learning and Memory
    • G-protein-coupled Receptors & Ion Channels
    • Post-mortem Brain Tissue
    Other
    +
    • Circular RNA
    • Gene Fusions
    • HT Transcript Validation
    • Long Non-coding RNA
    • RNAseq Validation
    • Single Cell Analysis
    • Splice Variant
    • miRNA
    RNA & Protein
    +
    • Antibody Challenges
    • Dual ISH + IHC Methods
    • No Antibodies
    • RNA & Protein Analysis
    Customer Innovations
    +
    • Dual RNA+DNA ISH
    • Very old FFPE ISH
    • Wholemount ISH
    Animal Models
    +
    • Any Species
    • Mouse Model
    • Preclincal Safety
  • Technology +
    Overview
    +
    • How it Works
    • Data Image Gallery
    • Technology Video
    • Webinars
    RNA Detection
    +
    • Why RNA?
    • RNA ISH and IHC
    Pretreatment Options
    +
    • RNAscope™ Pretreatment
    • PretreatPro™
    Spotlights
    +
    • Researchers Spotlights
    • RNA & DNA
    • WISH
    • FFPE
    • Testimonials
    Publications, Guides & Posters
    +
    • Search publications
    • RNAscope™ Reference Guide
    • RNAscope™ Data Analysis Guide
    • Download RNAscope™ Posters
  • Support +
    Overview
    +
    • Get Started
    • How to Order
    • Distributors
    • Contact Support
    Troubleshooting
    +
    • Troubleshooting Guide
    • FAQs
    • User Manuals, SDS and Product Inserts
    • Documents and Downloads
    Imaging Resource
    +
    • Image Analysis
    • Image Registration Software
    • QuPath
    • HALO® image analysis platform
    Learn More
    +
    • Webinars
    • Training Videos
  • Partners +
    Partners
    +
    • Overview
    Partners Directory
    +
    Automation Partners
    • Leica Biosystem
    • Roche Diagnostics
    Workflow Partners
    • NanoString
    Software Partners
    • indica labs
    Become a Partner
    +
    • Learn How
  • Diagnostics +
    Diagnostics
    +
    • Diagnostics
    • Literature
    • Diagnostics ASR Probes
    • Diagnostics CE-IVD Probes
    • Diagnostics CE-IVD Detection
    • Companion Diagnostics
  • Image Calendar +
    Image Calendar
    +
    • Image Contest
    • Data Image Gallery
Search

Probes for PPIB

ACD can configure probes for the various manual and automated assays for PPIB for RNAscope Assay, or for Basescope Assay compatible for your species of interest.

Your search for "PPIB" returned results. Search for our Top genes LGR5, vglut2, gad67, brca1

    Refine Probe List

    Content for comparison

    RNAscope™ HiPlex CS Probe - V-Powassan-T12

    Gene

    • PPIB (17) Apply PPIB filter
    • DapB (5) Apply DapB filter
    • POLR2A (4) Apply POLR2A filter
    • CD274 (1) Apply CD274 filter
    • Dkk3 (1) Apply Dkk3 filter
    • Wnt4 (1) Apply Wnt4 filter
    • Axin2 (1) Apply Axin2 filter
    • Fgfr3 (1) Apply Fgfr3 filter
    • VTN (1) Apply VTN filter
    • GAPDH (1) Apply GAPDH filter
    • Rspo3 (1) Apply Rspo3 filter
    • CDKN2A (1) Apply CDKN2A filter
    • KRT19 (1) Apply KRT19 filter
    • CSF1 (1) Apply CSF1 filter
    • ETV5 (1) Apply ETV5 filter
    • TSLP (1) Apply TSLP filter
    • FOS (1) Apply FOS filter
    • Lgr5 (1) Apply Lgr5 filter
    • GFAP (1) Apply GFAP filter
    • IL33 (1) Apply IL33 filter
    • LIN28A (1) Apply LIN28A filter
    • PECAM1 (1) Apply PECAM1 filter
    • Spp1 (1) Apply Spp1 filter
    • Tbp (1) Apply Tbp filter
    • PAX6 (1) Apply PAX6 filter
    • (-) Remove UBC filter UBC (1)
    • CX3CL1 (1) Apply CX3CL1 filter
    • Foxa2 (1) Apply Foxa2 filter
    • Id4 (1) Apply Id4 filter
    • Wt1 (1) Apply Wt1 filter
    • Ace2 (1) Apply Ace2 filter
    • PDL1 (1) Apply PDL1 filter
    • Cd36 (1) Apply Cd36 filter
    • HR18 (1) Apply HR18 filter
    • Dpep1 (1) Apply Dpep1 filter
    • IGHM (1) Apply IGHM filter
    • Chad (1) Apply Chad filter
    • ARX (1) Apply ARX filter
    • Zic1 (1) Apply Zic1 filter
    • CXCL8 (1) Apply CXCL8 filter
    • Mia (1) Apply Mia filter
    • Cdr1as (1) Apply Cdr1as filter
    • c-MET (1) Apply c-MET filter
    • CLEC3B (1) Apply CLEC3B filter
    • Slc25a47 (1) Apply Slc25a47 filter
    • MEOX1 (1) Apply MEOX1 filter
    • IGHG3 (1) Apply IGHG3 filter
    • SARS-CoV-2 (1) Apply SARS-CoV-2 filter
    • Bhmt (1) Apply Bhmt filter
    • (-) Remove Gad1 (Gad67) filter Gad1 (Gad67) (1)

    Product

    • RNAscope Multiplex Fluorescent Assay (2) Apply RNAscope Multiplex Fluorescent Assay filter

    Research area

    • Neuroscience (1) Apply Neuroscience filter

    Category

    • Publications (2) Apply Publications filter
    Expression of immunoglobulin constant domain genes in neurons of the mouse central nervous system

    Life science alliance

    2021 Nov 01

    Scheurer, L;Das Gupta, RR;Saebisch, A;Grampp, T;Benke, D;Zeilhofer, HU;Wildner, H;
    PMID: 34433614 | DOI: 10.26508/lsa.202101154

    General consensus states that immunoglobulins are exclusively expressed by B lymphocytes to form the first line of defense against common pathogens. Here, we provide compelling evidence for the expression of two heavy chain immunoglobulin genes in subpopulations of neurons in the mouse brain and spinal cord. RNA isolated from excitatory and inhibitory neurons through ribosome affinity purification revealed Ighg3 and Ighm transcripts encoding for the constant (Fc), but not the variable regions of IgG3 and IgM. Because, in the absence of the variable immunoglobulin regions, these transcripts lack the canonical transcription initiation site used in lymphocytes, we screened for alternative 5' transcription start sites and identified a novel 5' exon adjacent to a proposed promoter element. Immunohistochemical, Western blot, and in silico analyses strongly support that these neuronal transcripts are translated into proteins containing four Immunoglobulin domains. Our data thus demonstrate the expression of two Fc-encoding genes Ighg3 and Ighm in spinal and supraspinal neurons of the murine CNS and suggest a hitherto unknown function of the encoded proteins.
    Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide

    Cells

    2021 May 21

    Xiao, L;Labaer, J;Guo, J;
    PMID: 34063986 | DOI: 10.3390/cells10061277

    Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations. However, the current multiplexed RNA imaging technologies are limited by their relatively low sensitivity or specificity, which hinders their applications in studying highly autofluorescent tissues, such as formalin-fixed paraffin-embedded (FFPE) tissues. To address this issue, here we develop a multiplexed in situ RNA profiling approach with a high sensitivity and specificity. In this approach, transcripts are first hybridized by target-specific oligonucleotide probes in pairs. Only when these two independent probes hybridize to the target in tandem will the subsequent signal amplification by oligonucleotide hybridization occur. Afterwards, horseradish peroxidase (HRP) is applied to further amplify the signal and stain the target with cleavable fluorescent tyramide (CFT). After imaging, the fluorophores are chemically cleaved and the hybridized probes are stripped by DNase and formamide. Through cycles of RNA staining, fluorescence imaging, signal cleavage, and probe stripping, many different RNA species can be profiled at the optical resolution. In applying this approach, we demonstrated that multiplexed in situ RNA analysis can be successfully achieved in both fixed, frozen, and FFPE tissues.
    X
    Description
    sense
    Example: Hs-LAG3-sense
    Standard probes for RNA detection are in antisense. Sense probe is reverse complent to the corresponding antisense probe.
    Intron#
    Example: Mm-Htt-intron2
    Probe targets the indicated intron in the target gene, commonly used for pre-mRNA detection
    Pool/Pan
    Example: Hs-CD3-pool (Hs-CD3D, Hs-CD3E, Hs-CD3G)
    A mixture of multiple probe sets targeting multiple genes or transcripts
    No-XSp
    Example: Hs-PDGFB-No-XMm
    Does not cross detect with the species (Sp)
    XSp
    Example: Rn-Pde9a-XMm
    designed to cross detect with the species (Sp)
    O#
    Example: Mm-Islr-O1
    Alternative design targeting different regions of the same transcript or isoforms
    CDS
    Example: Hs-SLC31A-CDS
    Probe targets the protein-coding sequence only
    EnEmProbe targets exons n and m
    En-EmProbe targets region from exon n to exon m
    Retired Nomenclature
    tvn
    Example: Hs-LEPR-tv1
    Designed to target transcript variant n
    ORF
    Example: Hs-ACVRL1-ORF
    Probe targets open reading frame
    UTR
    Example: Hs-HTT-UTR-C3
    Probe targets the untranslated region (non-protein-coding region) only
    5UTR
    Example: Hs-GNRHR-5UTR
    Probe targets the 5' untranslated region only
    3UTR
    Example: Rn-Npy1r-3UTR
    Probe targets the 3' untranslated region only
    Pan
    Example: Pool
    A mixture of multiple probe sets targeting multiple genes or transcripts

    Enabling research, drug development (CDx) and diagnostics

    Contact Us
    • Toll-free in the US and Canada
    • +1877 576-3636
    • 
    • 
    • 
    Company
    • Overview
    • Leadership
    • Careers
    • Distributors
    • Quality
    • News & Events
    • Webinars
    • Patents
    Products
    • RNAscope or BaseScope
    • Target Probes
    • Controls
    • Manual assays
    • Automated Assays
    • Accessories
    • Software
    • How to Order
    Research
    • Popular Applications
    • Cancer
    • Viral
    • Pathways
    • Neuroscience
    • Other Applications
    • RNA & Protein
    • Customer Innovations
    • Animal Models
    Technology
    • Overview
    • RNA Detection
    • Spotlight Interviews
    • Publications & Guides
    Assay Services
    • Our Services
    • Biomarker Assay Development
    • Cell & Gene Therapy Services
    • Clinical Assay Development
    • Tissue Bank & Sample Procurement
    • Image Analysis
    • Your Benefits
    • How to Order
    Diagnostics
    • Diagnostics
    • Companion Diagnostics
    Support
    • Getting started
    • Contact Support
    • Troubleshooting Guide
    • FAQs
    • Manuals, SDS & Inserts
    • Downloads
    • Webinars
    • Training Videos

    Visit Bio-Techne and its other brands

    • bio-technie
    • protein
    • bio-spacific
    • rd
    • novus
    • tocris
    © 2025 Advanced Cell Diagnostics, Inc.
    • Terms and Conditions of Sale
    • Privacy Policy
    • Security
    • Email Preferences
    • 
    • 
    • 

    For Research Use Only. Not for diagnostic use. Refer to appropriate regulations. RNAscope is a registered trademark; and HybEZ, EZ-Batch and DNAscope are trademarks of Advanced Cell Diagnostics, Inc. in the United States and other countries. All rights reserved. ©2025 Advanced Cell Diagnostics, Inc.

     

    Contact Us / Request a Quote
    Download Manuals
    Request a PAS Project Consultation
    Order online at
    bio-techne.com
    OK
    X
    Contact Us

    Complete one of the three forms below and we will get back to you.

    For Quote Requests, please provide more details in the Contact Sales form below

    • Contact Sales
    • Contact Support
    • Contact Services
    • Offices

    Advanced Cell Diagnostics

    Our new headquarters office starting May 2016:

    7707 Gateway Blvd.  
    Newark, CA 94560
    Toll Free: 1 (877) 576-3636
    Phone: (510) 576-8800
    Fax: (510) 576-8798

     

    Bio-Techne

    19 Barton Lane  
    Abingdon Science Park
    Abingdon
    OX14 3NB
    United Kingdom
    Phone 2: +44 1235 529449
    Fax: +44 1235 533420

     

    Advanced Cell Diagnostics China

    20F, Tower 3,
    Raffles City Changning Office,
    1193 Changning Road, Shanghai 200051

    021-52293200
    info.cn@bio-techne.com
    Web: www.acdbio.com/cn

    For general information: Info.ACD@bio-techne.com
    For place an order: order.ACD@bio-techne.com
    For product support: support.ACD@bio-techne.com
    For career opportunities: hr.ACD@bio-techne.com

    See Distributors
    ×

    You have already Quick ordered an Item in your cart . If you want to add a new item , Quick ordered Item will be removed form your cart. Do You want to continue?

    OK Cancel
    Need help?

    How can we help you?