RNAscope™ LS Assay is compatible on the Leica Biosystems BOND RX automated IHC/ISH slide staining systems.

RNAscope™ LS (Leica Biosystems) Multiplex assay is a RNA in situ hybridization (ISH) fluorescent assay for use of three fluorescent dyes for single plex, duplex and up to 3-plex capability with the Leica Biosystems BOND RX automated IHC/ISH slide staining systems (Note: Using Leica Software v BDZ 11). The kit includes the pretreatment, TSA buffer (322810), and detection reagents for automated amplification. Each reagent kit provides enough reagents to stain ~60 slides. Leica Research Kit (DS9455) , is also required to run the RNAscope Multiplex Fluorescent Kit.

RNAscope™ LS 4-Plex Ancillary kit is designed to work with RNAscope LS Multiplex Flourescent Reagent Kit (322800) for 4 plex fluorescent in situ hybridization. It contains C4 HRP blocker and TSA buffer. Note: Opal fluorophores from Akoya Biosciences must be purchased separately (Opal 520, PN FP1487001KT; Opal 570, PN FP1488001KT; Opal 620, PN FP1495001KT; Opal 690, PN FP1497001KT). Multi spectral imaging systems are required for adequate imaging of 4 plex fluorescent images

RNAscope™2.5 LS (Leica Biosystems) assay is a RNA in situ hybridization (ISH) single-plex, chromogenic -RED assay for use with the Leica Biosystems BOND RX automated IHC/ISH slide staining systems. The automated system provides high throughput, reproducible RNA ISH results with minimal hands-on time. RNAscope™Leica Reagent Kit, includes the pretreatment and detection reagents for automated amplification.

RNAscope™ 2.5 LS (Leica Biosystems) assay is a RNA in situ hybridization (ISH) single-plex, chromogenic -BROWN assay for use with the Leica Biosystems BOND RX automated IHC/ISH slide staining systems. The automated system provides high throughput, reproducible RNA ISH results with minimal hands-on time. RNAscope™Leica Reagent Kit, includes the pretreatment and detection reagents for automated amplification. Each reagent kit provides enough reagents to stain ~60 slides/tissue sections, each with an area of approximately 20 mm x 20 mm (0.75inx 0.75in).

RNAscope™ 2.5 LS Green Accessory Pack includes ACD?s Green chromagen and Wash buffer (PN 310091). Note please purchase Vectamount (PN 321584) is required and must be purchased separately.

RNAscope™ 2.5 LS (Leica Biosystems) Duplex assay is a RNA in situ hybridization (ISH) duplex, chromogenic -(RED/BROWN) assay for use with the Leica Biosystems BOND RX automated IHC/ISH slide staining systems. The automated system provides high throughput, reproducible RNA ISH results with minimal hands-on time. RNAscope™Leica Reagent Kit, includes the pretreatment and detection reagents for automated amplification. Each reagent kit provides enough reagents to stain ~60 slides/tissue sections (20 slides or two trays per run), each with an area of approximately 20 mm x 20 mm (0.75inx 0.75in).