"Single Cell RNA-sequencing (scRNA-seq): A Practical Guideline"

In the last decade, single cell RNA-sequencing (scRNA-seq) technology has helped understand the underlying heterogeneity of different complex tissue systems, and identify rare cell populations, which would, otherwise, remain undiagnosed. The technology itself has evolved remarkably in this time- starting from low-throughput plate-based approach to microfluidic-assisted droplet-based high-throughput platforms and now making steady progress towards multi-omics. The requirement of high-quality samples poses a great deal of challenge to the researchers.  The Single Cell Core at Harvard Medical School, from their extensive experience of working with many different tissues across various organisms, has developed the best practices for preparing good quality samples compatible with scRNA-seq. Mandovi Chatterjee, the Deputy Director of the core, will share a practical guideline for a successful single cell experiments in her talk.