Nucleic acid-based molecular detection methods have revolutionized viral detection, offering several essential advantages such as sensitivity, specificity and speed. Direct detection of viral RNA in human or other animal cells by RNA in situ hybridization (ISH) is a powerful tool to establish the etiology and pathogenesis of viral disease.
We will discuss detection of AAV and LV vector biodistribution and transgene expression in preclinical animal models with RNAscope™ and BaseScope™ in situ hybridization. RNAscope in situ assays of AAV DNA and transgene mRNA combine the molecular sensitivity of qPCR with single-cell resolution and the context of tissue morphology. BaseScope enables specific detection of even single nucleotide differences and can easily differentiate AAV human transgene from non-human primate and other animal ortholog sequences.
In this webinar, we will demonstrate how ACD’s assay service offerings can be leveraged to detect viral infection and pathogenesis, and enhance our understanding of globally important viruses, such as SARS-CoV-2.
- Detection of positive single-stranded RNA viruses (Zika, MERS-CoV, hepatitis A/C/E, coronavirus) using RNAscope technology
- Visualization of the SARS-Cov-2 viral RNA using the V-nCoV2019-S probe and detect viral replication with the V-nCoV2019-S sense
- Simultaneous AAV vector biodistribution and transgene mRNA expression analysis
- Localize specific cell types and tissues infected with HIV/SIV – ideal for identifying and measuring persistent reservoirs with single-cell resolution in full morphological context
