2017 Mar 21 - Application of combined RNA in situ hybridization and multiplex immunohistochemistry to unravel tumor heterogeneity in prostate cancer
Nallasivam Palanisamy, PhD
Associate Scientist, Henry Ford Health System
Courtney Anderson, PhD
Senior Scientist, R&D, Advanced Cell Diagnostics Registration
Outline:

Prostate cancer is a complex disease with multiple tumors originating independently at different stages of growth. Despite the identification of key morphological differences between individual tumor foci, the underlying molecular mechanisms driving growth within individual foci are often poorly understood. This is because traditional molecular and genetic studies involving systemic sampling of large tumor foci or high Gleason grade tumor foci often miss small tumor foci with important driver aberrations and high metastatic potential. To avoid overlooking foci with important driver aberrations, well-characterized cancer-specific markers can be used to screen the entire prostate tissue to assess molecular differences in individual tumor foci. A clearer understanding of foci-specific molecular heterogeneity is important, as these molecular differences may prognosticate tumor growth and treatment success. 

In this presentation, we will demonstrate the power of dual immunohistochemistry (IHC) and in situ hybridization (ISH) as a technique to probe multiple cancer-specific molecular markers in the morphological context. Combining the protein detection capabilities of IHC with single-molecule mRNA expression analysis using RNAScope™ ISH technology builds a more complete and robust picture of the molecular mechanisms governing individual tumor foci. In addition to highlighting the development of dual IHC/ISH to characterize solid tumor heterogeneity at the molecular level, we will present how high-sensitivity, single RNA molecule detection ISH combined with IHC can be applied to other biological questions to provide valuable information on the expression of molecular markers in nearly any biological pathway.

Learning Objectives:
  • Applying dual IHC/ISH methods
  • Understanding tumor heterogeneity
Dates and Registration:
March
21
10:00 AM PT
1:00 PM ET
5 PM GMT
6 PM CET
Register Now
X
Contact Us

Complete one of the three forms below and we will get back to you.

For Quote Requests, please provide more details in the Contact Sales form below

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Bio-Techne

19 Barton Lane  
Abingdon Science Park
Abingdon
OX14 3NB
United Kingdom
Phone 2: +44 1235 529449
Fax: +44 1235 533420

 

Advanced Cell Diagnostics China

20F, Tower 3,
Raffles City Changning Office,
1193 Changning Road, Shanghai 200051

021-52293200
info.cn@bio-techne.com
Web: www.acdbio.com/cn

For general information: Info.ACD@bio-techne.com
For place an order: order.ACD@bio-techne.com
For product support: support.ACD@bio-techne.com
For career opportunities: hr.ACD@bio-techne.com