Trouble Shooting

Antigen retrieval conditions often require optimization in both RNAscope and IHC, depending on the tissue type and how the sample was fixed and processed. We recommend optimizing conditions for the RNAscope Assay if tissue samples are not prepared according to the ACD recommended guideline of fixing samples in fresh 10% NBF for 16–32 hours.

The main differences compared to an IHC workflow are the following:

RNAscope Technology is a novel in situ hybridization (ISH) assay that detects target RNA within intact cells. The assay is based on a patented signal amplification and background suppression technology, representing a major advance over traditional RNA ISH. RNAscope Assays do not require an RNase-free environment.