RNAscope™ Intro Pack 2.5 HD Duplex Reagent Kit is recommended for new users getting started with RNAscope duplex assay on mouse samples. It includes required materials such as: RNAscope 2.5 HD Duplex Reagent Kit (PN 322430), Control Slide Pack -3T3 Cell Pellet (PN 310023),Hydrophobic Barrier Pen (PN 310018), Wash Buffer (310091), Duplex Positive Control probe Mm PPIB - C1 & Polr2A-C2(PN 321651), 2 Plex Negative Control Probe DapB-C1/ DapB-C2 (PN 320751) and Vectamount (PN 321584). Users can use the control slides and the probes, to get familiar with the assay workflow and staining results