RNAscope® Technology Seminar - London, UK

RNASCOPE® TECHNOLOGY 

March 29, 2017 

12.30 to 13.30

Institute of Reproductive and Developmental Biology (IRDB)
Imperial College London
Du Cane Road, London

Ground Floor Seminar Room


 

The RNAscope® technology is being routinely used for tissue-based gene expression analysis for target validation, pathobiology and biomarker development in every disease area. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS.

 

The seminar will give an overview on:

Visualizing gene expression and genetic variations in tissue environments by RNAscope® and BaseScope™

  • Detect mRNAs and lncRNAs at single molecule sensitivity by RNAscope® assay
  • Target specific splice junctions for aberrant alternative splicing in cancers by BaseScope™ assay
  • Identify point mutations or gene editing by BaseScope™ assay
  • Distinguish specific homologues, paralogues, and orthologues by RNAscope® assay or BaseScope™ assay
  • Perform single cell analysis with morphological information in FFPE or frozen tissue
  • Pinpoint expression of novel RNAs of interest 


Comprehensive evaluation of the tumor-immune environment by RNAscope® and BaseScope™

  • Characterize expression profiles of various checkpoint markers and therapeutic targets in the tumor microenvironment (TME)
  • Carry out quantitative analysis of co-expression of multiple checkpoint molecules within the same cell
  • Evaluate immune function by visualizing mRNA expression of critical molecules including IFNg and other cytokines and chemokines in the TME
  • Assess the distribution of specific T cell clones, detected by specific CDR sequences, in tumoral and stromal environments
  • Go beyond the previous restriction of short sequence limitations
Location: Institute of Reproductive and Developmental Biology (IRDB), Imperial College London, Du Cane Road, London

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