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2021 July 27: Introducing the RNAscope HiPlex v2.0 assay for single-cell and spatial transcriptomic profiling.
Anushka Dikshit, Ph.D.
Applications Scientist, Genomics, Advanced Cell Diagnostics
Watch Video:
Purvi Shah
Sr. Product Manager, Genomics, Advanced Cell Diagnostics
Outline:

Introducing the RNAscope™ HiPlex v2.0 assay for single cell and spatial transcriptomic profiling of complex tissues using FFPE and frozen samples. The RNAscope™ in situ hybridization (ISH) technology provides a powerful method to detect gene expression with spatial and morphological context. The proprietary “double Z” probe design in combination with the advanced signal amplification enables highly specific and sensitive detection of the target RNA with each dot representing a single RNA transcript. The new HiPlex v2 assay expands the capabilities of the existing HiPlex assay and can now be used for simultaneous detection of 12 targets in FFPE tissues in addition to fixed frozen and fresh frozen tissues. This webinar will demonstrate detection and characterization of tumor infiltrating immune cells using the new HiPlex v2 assay thereby advancing our understanding of the dynamic crosstalk within the complex and heterogeneous tumor microenvironment (TME)

Learning Objectives:
  • Learn about the expanded capabilities and applications of the HiPlex v2 assay
  • Understand how the assay enables characterization of immune cell types and activation states
  • Study complex spatial relationships among different cell types within the TME
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