RNA is a key biomarker of the dynamic gene expression changes occurring in cells and tissues. Compared to techniques that analyze bulk tissue, identifying RNA expression at the single cell level within the morphological context provides valuable spatial information on gene expression. In this webinar we will present the advanced single-cell, single-molecule RNA in situ hybridization (ISH) technology RNAscope. We will discuss the technology, how to perform the assay, how to analyze and quantify the results, and provide multiple applications of the technology in both academic and clinical research settings.