About RNAscope® Technology
Although in situ RNA detection techniques (ISH and FISH) have been around for years, they are largely ineffective. They lack robustness and sensitivity to reliably detect the expression of most human genes. Microarray and PCR are gold standard molecular methods for disease profiling, but clinically relevant information regarding cellular and tissue context, as well as spatial variation of the expression patterns, is lost in the process.
Inspired to solve these challenges, ACD scientists revolutionized the RNA in situ hybridization method and in 2011 introduced RNAscope® ISH for detection of target RNA within intact cells. The assay represents a major advance in RNA ISH approaches with its proprietary probe design that simultaneously amplify target-specific signals and suppress background noise from non-specific hybridization. Known for its sensitivity and specificity, RNAscope® ISH is now a proven technology that has been featured in over 500 publications across a multitude of research areas. ACD offers both comprehensive Pharma Assay Services and Products for the assay to be conducted in research labs-- kits, accessories and catalog probes for over 9,000 targets in over 140 species.
Get familiar with RNAscope® ISH:
RNAscope® in situ hybridization assays are applicable to a wide variety of research applications. Browse our application library to learn how you can use RNAscope® technology to better understand disease pathology, develop new research tools, and visualize signaling pathways in situ.
Why use RNAscope® ISH?
New advances in RNA in situ hybridization transforms molecular detection
with morphological context enabling scientists to extract new data dimensions
Dr. Patricia Pentavento
"We have been using RNAscope® ISH, and have obtained a very sensitive detection with essentially no background. Both Sales and Support staffs have been exceptional in providing assistance at all stages of the process, getting us from concept to labeled slide with timely and expert advice."
RNAscope® technology has grown at an uprecedented rate - over 580 pulications since 2011. Review, search or download the completed peer-reviewed publication list.