Wnt / β-Catenin Signaling Pathway

Expression of REST in Human ovarian cancer sample using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

Expression of REST in Human ovarian cancer sample using RNAscope® 2.5 HD Assay Brown

WNT/β-catenin signaling pathway expression analysis RNA Expression CTNNB1 gene human renal carcinoma RNAscope LS 2.5 HD Brown Assay

RNA Expression of CTNNB1 in human renal carcinoma using RNAscope LS 2.5 HD Brown Assay

RNA expression of Wnt4 (green) and Axin2 (red) (WNT B-Catenin signaling pathway) in mouse skin using RNAscope<sup>®</sup> Duplex Assay.

RNA expression of Wnt4 (green) and Axin2 (red) in mouse skin using RNAscope® Duplex Assay.

RNA expression of REST gene (WNT B-Catenin signaling pathway) in human esophageal cancer sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of REST gene in human esophageal cancer sample using RNAscope® 2.5 HD Brown Assay

RNA expression of REST gene (WNT B-Catenin signaling pathway) in human glioma sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of REST gene in human glioma sample using RNAscope® 2.5 HD Brown Assay

RNAscope<sup>®</sup> 2.5 - Mouse Pancreas - Wnt4 (WNT B-Catenin signaling pathway) in situ hybridization

RNAscope® 2.5 - Mouse Pancreas - Wnt4

RNA expression of REST gene (WNT B-Catenin signaling pathway) in human liver cancer sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of REST gene in human liver cancer sample using RNAscope® 2.5 HD Brown Assay

RNA expression of REST gene (WNT B-Catenin signaling pathway) in human meningioma sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of REST gene in human meningioma sample using RNAscope® 2.5 HD Brown Assay

RNA expression of JUN gene in human ovarian cancer TMA sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of JUN gene in human ovarian cancer TMA sample using RNAscope® 2.5 HD Brown Assay

RNA expression of JUN gene in human esophageal cancer TMA sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of JUN gene in human esophageal cancer TMA sample using RNAscope® 2.5 HD Brown Assay

RNA expression of MYC gene (WNT B-Catenin signaling pathway) in human bladder cancer sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of MYC gene in human bladder cancer sample using RNAscope® 2.5 HD Brown Assay

RNA expression of MYC gene (WNT B-Catenin signaling pathway) in human cervical cancer sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of MYC gene in human cervical cancer sample using RNAscope® 2.5 HD Brown Assay

RNA expression of MYC gene (WNT B-Catenin signaling pathway) in human colorectal cancer sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of MYC gene in human colorectal cancer sample using RNAscope® 2.5 HD Brown Assay

RNA expression of MYC gene (WNT B-Catenin signaling pathway) in human liver cancer sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of MYC gene in human liver cancer sample using RNAscope® 2.5 HD Brown Assay

RNA expression of GSK3B gene (WNT B-Catenin signaling pathway) in human esophageal cancer TMA sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of GSK3B gene in human esophageal cancer TMA sample using RNAscope® 2.5 HD Brown Assay

RNA expression of GSK3B gene (WNT B-Catenin signaling pathway) in human ovarian cancer TMA sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of GSK3B gene in human ovarian cancer TMA sample using RNAscope® 2.5 HD Brown Assay

WNT/β-catenin signaling pathway expression analysis RNA expression of GSK3A in human esophageal cancer RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of GSK3A gene in human esophageal cancer TMA sample using RNAscope® 2.5 HD Brown Assay

RNA expression of GSK3A gene (WNT B-Catenin signaling pathway) in human ovarian cancer TMA sample using RNAscope<sup>®</sup> 2.5 HD Brown Assay

RNA expression of GSK3A gene in human ovarian cancer TMA sample using RNAscope® 2.5 HD Brown Assay

RNAscope<sup>®</sup> 2.5 Brown - Mouse Eye - Wnt4 (WNT B-Catenin signaling pathway)

RNAscope® 2.5 Brown - Mouse Eye - Wnt4

WNT B-Catenin Pathway mRNAs

Lim et al (2016) PNAS 16599113 Figure 3

WNT B-Catenin Pathway

 

Pathway information and image are provided courtesy of R&D Systems, a Bio-Techne Brand. A high resolution and interactive image of this pathway is available on their website

 

WNT/ β-catenin signaling is involved in a variety of cellular processes related to development, including differentiation, homeostasis, genetic stability, and proliferation.  Aberrant signaling of the WNT/ β-catenin pathway is also associated with many types of cancer. Secreted proteins, like WNTs, are challenging for antibody detection using immunohistochemistry (IHC) and require alternative methods to identify their cellular localization. The RNAscope® in situ hybridization (ISH) assay provides single-cell gene expression resolution with spatial and morphological context.

 

The RNAscope® ISH assay is ideal for expression analysis of the WNT/ β-catenin signaling pathway:

 

Proven ultra-high sensitivity for the detection of secreted proteins—Accurate detection of secreted proteins like WNTs require highly sensitive assays.  RNAscope ISH, known for its ultra-sensitivity, has been referenced in over 40 WNT signaling pathway publications.

ANY Gene and WNT family member—When analyzing this pathway, researchers typically examine the expression of multiple WNT genes.  RNAscope probes can be designed for virtually ANY GENE, enabling researchers to apply a single detection method across all members of the WNT family as well as other genes in the pathway.

ANY Species and model systems—Studies of the WNT/ β-catenin pathway are often performed not only in human but also in model organisms such as mouse, C. elegans, Xenopus, zebrafish, and Drosophila. RNAscope probes can be designed for virtually ANY SPECIES so examination of genes in the WNT/ β-catenin pathway across multiple model organisms can be conducted seamlessly.

RNAscope® WISH in embryos—The whole-mount in situ hybridization (WISH) methodology in zebrafish with the RNAscope assay  was published 2014 by Gross-Thebing, et al. . This customer innovation enables the study of the WNT/β-catenin pathway in whole-mount embryos from species such as Xenopus, zebrafish, and Drosophila.

BaseScope™ Assay and WNT/ β-catenin pathway mutations—ACD’s newly introduced BaseScope Assay enables detection of splice variants, mutations, and more within the tissue environment.  This assay offers researchers a unique method to examine mutations in WNT pathway genes in situ and more thoroughly understand their roles in developmental defects. 

Wnt / β-Catenin Signaling Pathway Genes

(Click on the name to access corresponding RNAscope® Catalog Probes)

 

 

 

** If your gene of interest is not listed or is listed above but there is no catalog probe targeting your species of choice,  ACD can design and manufacture new probes in 2 weeks.

Request New Probes

 

Related Publications

There are over 40 publications where WNT gene and RNAscope ISH are both referenced.

X
Contact Us

Complete one of the two forms below and we will get back to you.

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Advanced Cell Diagnostics , S.r.l.

Via Calabria15
20090 Segrate (Milano) - Italy

Phone: +39.02.95360323
Fax: +39.02.95360992
For European orders: order_europe@acdbio.com

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china@acdbio.com
For general information: Leon Li lli@acdbio.com
Web: www.acdbio.com.cn

For general information: info@acdbio.com
For place an order: order@acdbio.com
For product support: support@acdbio.com
For career opportunities: hr@acdbio.com