Zika Virus Research
Detection of Zika viral genomic RNA with RNAscope probe #464531 (red punctate dots). The Vero cells sample was infected with ZIKV, MR766 strain. Data courtesy of Dr. Noriko Nakajima, National Institute of Infectious Disease, Japan
Detection of Zika viral cRNA (- strand) with RNAscope® probe #464541 ( red punctate dots). The Vero cells sample was infected with ZIKV, MR766 strain. Data courtesy of Dr. Noriko Nakajima, National Institute of Infectious Disease Japan
As a result of the current Zika virus outbreak, researchers are accelerating research into the biology of the virus and the pathology of human infection with the goals of understanding the causes of the clinical syndrome, developing reliable detection methods, and finding effective treatments.
Why use RNAscope® assay for zika virus research?
RNAscope® ISH, an innovated ISH assay, offers researchers a robust and accurate method to detect even low levels of viral RNA in virtually any tissue samples. The ability to detect the virus within the context of the infected tissue will advance the understanding of the zika viral biology.
Morphologic context—Performed in situ, RNAscope® assay preserves the morphology of the infected tissues, enabling detection of the viral RNA in the morphological context. Miner et al., 2016 demonstrated this in detection of Zika in eyes of mouse fetuses
Proven for viral detection—Numerous publications citing the use of RNAscope® in situ hybridization in viral studies spanning HPV, EPV, HIV, SIV, HCV, MERS etc. RNAscope® assay enables virologists to bypass time consuming and often non-yielding antibody development.
Proven for viral replication detection and localization-- read Bhatnagar J et al 2017
Versatile probe design for low viral count—When working with zika or other viruses of extremely low copy number, ACD can customize the probe design strategy to offset this challenge; for example by designing probes with more than the typical 20 Z probe pairs.
Specificity—The highly specific, proprietary RNAscope® double Z probe design, eliminates cross reactivity with other flavivirus RNA, enabling reliable differentiation from highly homologous viruses such as dengue.
Sensitivity—The ultrahigh sensitivity of RNAscope® assay and ability to detect single RNA molecules makes it ideal for in situ detection of low level tissue infection. Read Rosenberg et al (2017)