Learn more about our New BaseScopeTM assay
RNAscope® 2.5 LS Reagent Kit – RED
The RNAscope® 2.5 LS Assay is designed as an automated assay on the Leica Biosystems' BOND RX Research Advanced Staining System. The RNAscope® 2.5 LS Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH.
The RNAscope® 2.5 LS Reagent Kit-RED with it Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed, ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope.
These new RNAscope® 2.5 LS reagents require the new RNAscope® 2.5 LS Probes for optimal performance. Probe designs are identical to previous version, however the RNAscope® 2.5 LS probes are manufactured for optimal performance with RNAscope® 2.5 reagent chemistry.
RNAscope® 2.5 LS Red - Prostate Cancer - PPIB
RNAscope® 2.5 Red - Lung Cancer - POLR2A
RNAscope® 2.5 LS Red - Kidney Cancer - PPIB
RNAscope® 2.5 LS Red - Head & Neck Cancer - HPV16
The RNAscope® LS Automated Assay workflow requires the following from Leica Biosystems (LeicaBiosystems.com):
|CAT #||Product Name and Description||Quantity|
|OPT9049||BOND Titration Kit||10 containers, 50 inserts|
|OP79193||BOND Open Containers (7 mL)||10 pack|
|OP309700||BOND Open Containers (30 mL)||10 pack|
|S21.4611||BOND Universal Covertiles™||160 pack|
|AR9961 & AR9640||BOND Epitope Retrieval Solution 1 & 2||1L (RTU) each|
|S21.1971||BOND Mixing Stations||6 vials|
|AR9222||BOND Dewax Solution||1L (RTU)|
|AR9590||BOND Wash Solution (10x concentrate)||1L|
|DS9390||BOND Polymer Refine Red Detection||1 kit|
|CS9100||BOND Aspirating Probe Cleaning System||1 system, 15 cleans|
RNAscope® 2.5 LS Automated Assay—RED Workflow Requires the following from ACD:
- RNAscope® 2.5 LS Target Probes (Catalog or Made-to-Order LS Probes)
- RNAscope® 2.5 LS Control Probes (Select species-specific LS positive control probes or packs of probes and LS negative control probes)
- RNAscope® Control Slides (optional)
- RNAscope® 2.5 Leica Assay Reagent Kit—RED
Effective June 30th 2016, RNAscope 2.0 product line will be discontinued and no longer available as a catalog product. This includes the following: RNAscope 2.0 Brown (Cat No 310033 or 310035), RNAscope 2.0 Red (Cat No 310036 or 310034),RNAscope 2.0 LS Brown (Cat No 321100), RNAscope 2.0 LS Red (Cat No 321130), RNAscope 2.0 VS Brown (Cat No 320600),RNAscope 2.0 VS Red (Cat No 320610).
Please refer to the FAQ for more information about 2.0 discontinuance.
More information on RNAscope 2.5 can be viewed in the Webinar titled “RNAscope® New Product Development- learn more about RNAscope 2.5 product line” here