RNAscope® 2.5 LS Reagent Kit – RED

The RNAscope® 2.5 LS Assay is designed as an automated assay on the Leica Biosystems' BOND RX Research Advanced Staining System. The RNAscope® 2.5 LS Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH.

The RNAscope® 2.5 LS Reagent Kit-RED with it Fast Red dye offers a higher contrast and is the first choice in in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed,  ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope. 

These new RNAscope® 2.5 LS reagents require the new RNAscope® 2.5 LS Probes for optimal performance. Probe designs are identical to previous version, however the RNAscope® 2.5 LS probes are manufactured for optimal performance with RNAscope® 2.5 reagent chemistry.

Sample Images
RNAscope<sup>®</sup> 2.5 LS Red - Prostate Cancer - PPIB

RNAscope® 2.5 LS Red - Prostate Cancer - PPIB

RNAscope<sup>®</sup> 2.5 Red - Lung Cancer - POLR2A

RNAscope® 2.5 Red - Lung Cancer - POLR2A

RNAscope<sup>®</sup> 2.5 LS Red - Kidney Cancer - PPIB

RNAscope® 2.5 LS Red - Kidney Cancer - PPIB

RNAscope<sup>®</sup> 2.5 LS Red - Head & Neck Cancer - HPV16

RNAscope® 2.5 LS Red - Head & Neck Cancer - HPV16

The RNAscope® LS Automated Assay workflow requires the following from Leica Biosystems (LeicaBiosystems.com):

CAT # Product Name and Description Quantity
OPT9049 BOND Titration Kit 10 containers, 50 inserts
OP79193 BOND Open Containers (7 mL) 10 pack
OP309700 BOND Open Containers (30 mL) 10 pack
S21.4611 BOND Universal Covertiles™ 160 pack
AR9961 & AR9640 BOND Epitope Retrieval Solution 1 & 2 1L (RTU) each
S21.1971 BOND Mixing Stations 6 vials
AR9222 BOND Dewax Solution 1L (RTU)
AR9590 BOND Wash Solution (10x concentrate) 1L
DS9800 BOND Polymer Refine Detection 1 kit
CS9100 BOND Aspirating Probe Cleaning System 1 system, 15 cleans


RNAscope® 2.5 LS Automated Assay—RED Workflow Requires the following from ACD:

  • RNAscope® 2.5 LS Target Probes (Catalog or Made-to-Order LS Probes)
  • RNAscope® 2.5 LS Control Probes (Select species-specific LS positive control probes or packs of probes and LS negative control probes)
  • RNAscope® Control Slides (optional)
  • RNAscope® 2.5 Leica Assay Reagent Kit—RED

Product Retirement:

Effective June 30th 2016, RNAscope 2.0 product line will be discontinued and no longer available as a catalog product. This includes the following: RNAscope 2.0 Brown (Cat No 310033 or 310035), RNAscope 2.0 Red (Cat No 310036 or 310034),RNAscope 2.0 LS Brown (Cat No 321100), RNAscope 2.0 LS Red (Cat No 321130), RNAscope 2.0 VS Brown (Cat No 320600),RNAscope 2.0 VS Red (Cat No 320610).

Please refer to the FAQ for more information about 2.0 discontinuance.

More information on RNAscope 2.5 can be viewed in the Webinar titled “RNAscope® New Product Development- learn more about RNAscope 2.5 product line” here

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