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Aperio RNA ISH Algorithm by Leica Biosystems

Manual RNA ISH interpretation is time-consuming, subject to inter/intra-observer variability and typically employs semi-quantitative reads. The Aperio RNA ISH Algorithm from Leica Biosystems enables accurate counting of individual signals across the tissue, providing standardized, reproducible results. Validation testing has demonstrated the high correlation between the Aperio RNA ISH Algorithm and expert manual review, making it ideal for researchers who want to automate routine counting tasks.

Aperio RNA ISH Algorithm Features:

  • Quantify Single or Duplex signals
  • Identify subcellular location of signal (nucleus, cytoplasm)
  • Count individual cells or clusters
  • Quantitative and semi-quantitative outputs
  • Compatible with full Aperio Digital Pathology solution
Sample Images
Original tissue showing RNA ISH detection duplex red / green chromogens and hematoxylin nuclear counterstain. Masks show Aperio RNA ISH Algorithm performance, where tissue area that have no cells, light blue is cytoplasm and dark blue are nuclei. Detection of individual RNA signals are shown as Red spot = Red mask, Green spot = Green mask. Pink annotations indicate the detection of RNA signal clusters.

Original tissue showing RNA ISH detection duplex red / green chromogens and hematoxylin nuclear counterstain. Masks show Aperio RNA ISH Algorithm performance, where tissue area that have no cells, light blue is cytoplasm and dark blue are nuclei. Detection of individual RNA signals are shown as Red spot = Red mask, Green spot = Green mask. Pink annotations indicate the detection of RNA signal clusters.

Original tissue showing RNA ISH detection single-plex with red chromagen and hematoxylin nuclear counterstain. Masks show Aperio RNA ISH Algorithm performance, where grey is total tissue, light blue is cytoplasm and dark blue are nuclei. Detection of individual RNA signals are shown as Red spot = Red mask. Pink annotations indicate the detection of RNA signal clusters.

Original tissue showing RNA ISH detection single-plex with red chromagen and hematoxylin nuclear counterstain. Masks show Aperio RNA ISH Algorithm performance, where grey is total tissue, light blue is cytoplasm and dark blue are nuclei. Detection of individual RNA signals are shown as Red spot = Red mask. Pink annotations indicate the detection of RNA signal clusters.

Original tissue showing RNA ISH detection single-plex with brown chromagen and hematoxylin nuclear counterstain. Mask shows Aperio RNA ISH Algorithm performance, where grey is total tissue, light blue is cytoplasm and dark blue are nuclei. Detection of individual RNA signals are shown as Brown spot = Orange mask. Pink annotations indicate the detection of RNA signal clusters.

Original tissue showing RNA ISH detection single-plex with brown chromagen and hematoxylin nuclear counterstain. Mask shows Aperio RNA ISH Algorithm performance, where grey is total tissue, light blue is cytoplasm and dark blue are nuclei. Detection of individual RNA signals are shown as Brown spot = Orange mask. Pink annotations indicate the detection of RNA signal clusters.

Aperio RNA ISH Algorithm is compatible with the chromogenic RNAscope Manual and Automated Assays:

  • RNAscope® 2.0 HD Reagent Kit—BROWN
  • RNAscope® 2.0 HD Reagent Kit—RED
  • RNAscope® 2.5 HD Reagent Kit—BROWN
  • RNAscope® 2.5 HD Reagent Kit—RED
  • RNAscope® 2-Plex Reagent Kit
  • RNAscope® LS Reagent Kit—BROWN
  • RNAscope® LS Reagent Kit—RED
  • RNAscope® VS Reagent Kit—BROWN
  • RNAscope® VS Reagent Kit—RED

The Aperio RNA ISH Algorithm forms part of Leica Biosystems’ comprehensive and open platform for ISH research, including the BOND RX rapid staining system, Aperio digital pathology scanners, and powerful software for image management and analysis. For more information, visit LeicaBiosystems.com/Aperio.

 

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