Manual RNA ISH interpretation is time-consuming, subject to inter/intra-observer variability and typically employs semi-quantitative reads. The Aperio RNA ISH Algorithm from Leica Biosystems enables accurate counting of individual signals across the tissue, providing standardized, reproducible results. Validation testing has demonstrated the high correlation between the Aperio RNA ISH Algorithm and expert manual review, making it ideal for researchers who want to automate routine counting tasks.
Aperio RNA ISH Algorithm Features:
- Quantify Single or Duplex signals
- Identify subcellular location of signal (nucleus, cytoplasm)
- Count individual cells or clusters
- Quantitative and semi-quantitative outputs
- Compatible with full Aperio Digital Pathology solution