• Sep
    26
    Product Introduction: RNAscope® VS Universal HRP, AP and Duplex Assays
    Vanee Pho, Ph.D, Senior Product Manager, Advanced Cell Diagnostics, Inc.
    Adrian Murillo, Development Manager, Ventana Medical Systems, Part of Roche Diagnostics
    Product Introduction: RNAscope® VS Universal HRP, AP and Duplex Assays
    Vanee Pho, Ph.D
    Senior Product Manager, Advanced Cell Diagnostics, Inc.
    Adrian Murillo
    Development Manager, Ventana Medical Systems, Part of Roche Diagnostics
    Outline:

    Join us for a co-hosted webinar with Roche Tissue Diagnostics, CDx, DISCOVERY focusing on the next generation RNAscope® VS Universal HRP, AP and Duplex Assays. These assays are ideal for single-molecule detection of up to two RNA targets simultaneously within the same tissue on the Ventana DISCOVERY ULTRA system.

    This webinar will highlight the next generation in RNAscope® technology for the Ventana system.

    This will include: 

    • RNAscope® VS Universal HRP using DAB detection (Brown)
    • RNAscope® VS Universal HRP using AP detection (Red)
    • RNAscope® VS Duplex  (Red/ Brown)

    Advanced Cell Diagnostics will present the RNAscope® technology - the methodology, examples of data obtained.  Roche Tissue Diagnostics, CDx, DISCOVERY will introduce new protocols to enable ISH/IHC or ISH/IF on the same slide using the mRNA Universal Procedure from Ventana Medical Systems, Inc.

    This webinar will benefit both new and experienced RNAscope® technology users.

    Please bring your RNAscope® technology questions to the webinar and our team will answer them live by chat or you can also send in your questions in advance to support@acdbio.com.

    Dates and Registration:
    September
    26
    9AM PST
    12PM EST
    6PM CET
    Register Now

You can watch a video or download presentation of the past webinars.

Recorded Webinars

Follow a step-by-step visual guide to assist with running the RNAscope Manual Assay.

De-paraffinization

De-paraffinization is performed to ensure complete removal of the paraffin from FFPE samples to allow for the probes to penetrate the target RNA after adequate pretreatment.

Endogenous Peroxidase Blocking

RNAscope H2O2 step is performed during the RNAscope assay to block endogenous peroxidase enzyme activity to prevent hazy background after detection.Note: Pretreatment 1 refers to RNAscope H2O2 reagent and is the first pretreatment perfomed on your samples for Chromogenic assays.

Target Retrieval (Boiling)

Target Retrieval step is a heat induced epitope retrieval method that is necessary to reverse the cross-linking caused by the formalin fixation step. Note: Pretreatment 2 refers to Target Retrieval reagent.For an alternative steamer protocol refer to the appendix of User Manual Part 2 Brown and Red.

Protease Plus (Protease Digestion)

Protease Plus is a broad spectrum protease that is intended to permeabilize the samples adequately to allow the probes to reach the target mRNA.Note : Pretreament 3 refers to Protease Plus reagent.

Target Probe Hybridization

ACD provides properietary double "ZZ" oligo probes designed to hybridize to your specific RNA target 

Amplification (Amp1-Amp6) with Wash Step

RNAscope detection reagents amplify hybridization signals via sequential hybridization of amplifiers.

DAB Colormetric Reaction

Chromogenic detection is based on the enzyme substrate reaction which leads to the formation of an insoluble precipitate visualized in the form of punctate dots for the RNAscope assay.

Gill's Hematoxylin Counter Stain

Hematoxylin staining is a counterstain used to provide a contrast to better visualize the signal and to observe  the morphology of the sample and identify the localization of the signal.

Tissue Dehydration

The tissue dehydration step after the counterstaining step results in removal of excess moisture which provides better  tissue morphology and preservation of the signal.

Mounting

The final step in RNAscope after staining requires the use of  mounting media to adhere a coverslip to tissue section or cell smear. This helps protect the sample and the staining from physical damage and helps improve the clarity and contrast of an image during microscopy.

Download any of the following webinar presentation documents.

PresentationsDownload File
Ready Set Go Getting Started with RNAscope_May 12 2015

Ready Set Go Getting Started with RNAscope_Apr 14 2015

RNAscope Troubleshooting Tips_June 16 2015

RNAscope Troubleshooting Tips_July 14 2015

Contact ACD Technical Support

How can we help you?

Please contact us directly, by phone or email, for any technical assistance with the RNAscope® assay or relevant products.

Call Technical Support
1 (877) 576-3636
8:00 am - 6.00 pm PST
X
Contact Us

Complete one of the two forms below and we will get back to you.

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Advanced Cell Diagnostics , S.r.l.

Via Calabria15
20090 Segrate (Milano) - Italy

Phone: +39.02.95360323
Fax: +39.02.95360992
For European orders: order_europe@acdbio.com

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china@acdbio.com
For general information: Leon Li lli@acdbio.com
Web: www.acdbio.com.cn

For general information: info@acdbio.com
For place an order: order@acdbio.com
For product support: support@acdbio.com
For career opportunities: hr@acdbio.com