Research Solutions: Non-Coding RNA
RNA expression of TUG1 gene in Human Gastric cancer sample using RNAscope® 2.5 HD Assay Brown
RNA expression of PVT1 gene in Human Lymphoma sample using RNAscope® 2.5 HD Assay Brown
RNA expression of SCHLAP1 gene in Human Prostate cancer sample using RNAscope® 2.5 HD Assay Brown
Expression of H19 in Human ovarian cancer sample using RNAscope® 2.5 HD Assay Brown
Expression of MALAT1 in Human Liver cancer sample using RNAscope® 2.5 HD Assay Brown
Expression of HOTAIR in Human Esophageal cancer sample using RNAscope® 2.5 HD Assay Brown
Expression of HOTAIR RNA (brown dots) in human breast cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.0 HD Reagent Kit-BROWN
Expression of PCA3 RNA (brown dots) in human prostate cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.0 HD Reagent Kit-BROWN
In the most recent statistics from the GENCODE project (v25, August 2015), the human genome contains 25,823 non-coding RNA (ncRNA) genes, surpassing the number of protein-coding genes (19,815). Of the non-coding RNA species, some 30% (9,882) are less than 200 bases long, termed as small non-coding RNA. They comprise of transfer RNA (tRNA) and ribosomal RNA (rRNA), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNA. About 60% (15,941) of the non-coding RNAs are longer than 200 bases, and are operationally designated as long non-coding RNAs (lncRNAs). The functions of lncRNAs are still being characterized; their abundance and diversity add to the challenge. Some lncRNAs have been shown to regulate gene expression through a diversity of mechanisms and play important roles in chromatin modification (HOTAIR), transcriptional and post-transcriptional regulation (ZEB2). Dysregulation of lncRNA is being found to have relevance not only in tumorigenesis, but also to neurological, cardiovascular, developmental and other diseases.
The discovery of a previously unknown universe of lncRNAs has created an unprecedented demand for effective RNA in situ hybridization tools. Unlike protein coding genes, for which immunohistochemistry (IHC) and RNA in situ hybridization are complementary for mapping gene expression to specific cells in situ, long non-coding RNA gene expression can only be interrogated by RNA in situ hybridization. The generally lower expression levels of lncRNAs than their protein coding counterparts demand the highest sensitivity from RNA in situ hybridization methods. The single-molecule sensitivity and rapid assay development time (<2 weeks) of ACD’s RNAscope® technology make RNAscope® ideally suited for localizing lncRNAs expression to specific cell types and sub-cellular structures. RNAscope® in situ assays will undoubtedly accelerate lncRNA research and become an indispensable tool for lncRNA-based molecular diagnostics.
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A list with all lncRNA publications can be found here