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Research Solutions: Non-Coding RNA

Expression of MALAT1 in Human Liver cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

Expression of MALAT1 in Human Liver cancer tissue using RNAscope® 2.5 HD Assay Brown

Expression of HOTAIR in Human Esophageal cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

Expression of HOTAIR in Human Esophageal cancer tissue using RNAscope® 2.5 HD Assay Brown

RNA expression of TUG1 gene in Human Gastric cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

RNA expression of TUG1 gene in Human Gastric cancer tissue using RNAscope® 2.5 HD Assay Brown

RNA expression of PVT1 gene in Human Lymphoma tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

RNA expression of PVT1 gene in Human Lymphoma tissue using RNAscope® 2.5 HD Assay Brown

RNA expression of SCHLAP1 gene in human prostate cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

RNA expression of SCHLAP1 gene in human prostate cancer tissue using RNAscope® 2.5 HD Assay Brown

Expression of H19 in Human ovarian cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

Expression of H19 in Human ovarian cancer tissue using RNAscope® 2.5 HD Assay Brown

Expression of HOTAIR RNA (brown dots) in human breast cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.0 HD Reagent Kit-BROWN

Expression of HOTAIR RNA (brown dots) in human breast cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.0 HD Reagent Kit-BROWN

lncRNA expression of PCA3 RNA (brown dots) in human prostate cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.0 HD Reagent Kit-BROWN

Expression of PCA3 RNA (brown dots) in human prostate cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.0 HD Reagent Kit-BROWN

In the most recent statistics from the GENCODE project (v25, August 2015), the human genome contains 25,823 non-coding RNA (ncRNA) genes, surpassing the number of protein-coding genes (19,815). Of the non-coding RNA species, some 30% (9,882) are less than 200 bases long, termed as small non-coding RNA. They comprise of transfer RNA (tRNA) and ribosomal RNA (rRNA), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNA. About 60% (15,941) of the non-coding RNAs are longer than 200 bases, and are operationally designated as long non-coding RNAs (lncRNAs). The functions of lncRNAs are still being characterized; their abundance and diversity add to the challenge. Some lncRNAs have been shown to regulate gene expression through a diversity of mechanisms and play important roles in chromatin modification (HOTAIR), transcriptional and post-transcriptional regulation (ZEB2). Dysregulation of lncRNA is being found to have relevance not only in tumorigenesis, but also to neurological, cardiovascular, developmental and other diseases.

The discovery of a previously unknown universe of lncRNAs has created an unprecedented demand for effective RNA in situ hybridization tools. Unlike protein coding genes, for which immunohistochemistry (IHC) and RNA in situ hybridization are complementary for mapping gene expression to specific cells in situ, lncRNA expression can only be interrogated by RNA in situ hybridization. The generally lower expression levels of lncRNAs than their protein coding counterparts demand the highest sensitivity from RNA in situ hybridization methods. The single-molecule sensitivity and rapid assay development time (<2 weeks) of ACD’s RNAscope® technology make RNAscope® ideally suited for localizing lncRNAs expression to specific cell types and sub-cellular structures. RNAscope® in situ assays will undoubtedly accelerate lncRNA research and become an indispensable tool for lncRNA-based molecular diagnostics.

Some of the available RNAscope probes for lncRNA - Don’t see your gene of interest? RNAscope® Made-to-Order Target Probes and manufactured within 2 weeks for virtually ANY gene from ANY species in ANY tissue. Learn more at www.acdbio.com/target-probes-made-order

0610012G03Rik BREA2 EPOR LHFPL3-AS2 LncI22 MYCNOS RAD51-AS1
0610038B21Rik C230091D08Rik Fendrr LINC00152 LOC100132735 NAALADL2-AS3 RoR
1500009C09Rik CASC9 FLJ32255 LINC00162 LOC100506013 NBR2 RP11-255G12-lincRNA
1700019B21Rik CCAT1 G530011O06Rik LINC00302 LOC100507412 NCBP2-AS2 RP23-80I4.3
3110099E03Rik CDKN2B-AS1 GAS5 LINC00473 LOC100507506 NDRG4 SCHLAP1
4833428L15Rik charon-lnc GDNF-AS1-LongNcTv LINC00515 LOC100507639 Neat1 SNHG12
4930463O16Rik CHN1 Gm10125 LINC00538 LOC102636514 NR_015410 SNHG19
6430562O15Rik CLEC4GP1 Gm15850-LncRNA LINC00589 LOC157273 Nron Snhg7
9130024F11Rik CLU-tv3 Gm2694 LINC00704 LOC339535 Olfr1372 spaw-lnc
A330023F24Rik CPS1-IT1 Gm5083 LINC00707 LOC400043 P2RX7 TINCR
A930006I01Rik CRNDE Gpr19 LINC00839 LUCAT1 PCA3 TRERNA1
A930011O12Rik CTA-204B4.6 H19 LINC00844 MALAT1 PCAT1 Ttc39aos1
ADAMTS9-AS2 CYP3A5 HCP5 LINC00887 MEG3 PCAT14 TUG1
AJAXC1-ncRNA DANCR Hmga2-ps1 LINC00941 Meg3-tv3 PCGEM1 UCA1
AOC4P DBET HOTAIR LINC01133 MESTIT1 PRNCR1 UNK-ncRNA
Apela DISC2 HOXB-AS51 LINC01212 Mhrt NR_030682 WNT2
ATXN3 DLEU1 HOXC-AS3 LINC01296 MIAT PTENP1 XIST
BCAR4 DUX4L-X-family IL15 LINC01546 MIR155HG PVT1 Zeb2os
BCYRN1 E030019B13Rik KCNQ1OT1 Linc-KLF14-1 MIR503 PXN-AS1  
BIN3-IT1 EGFR-AS1 KDM4A-AS1 lnc-FAM164A-1 MNX1-AS1 RAD21-AS1  

 

A list with all lncRNA publications can be found here

Publications

Long non-coding RNA chromogenic in situ hybridisation signal pattern correlation with breast tumour pathology

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