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Research Solutions: Non-Coding RNA

Expression of HOTAIR in Human Esophageal cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

Expression of HOTAIR in Human Esophageal cancer tissue using RNAscope® 2.5 HD Assay Brown

RNA expression of TUG1 gene in Human Gastric cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

RNA expression of TUG1 gene in Human Gastric cancer tissue using RNAscope® 2.5 HD Assay Brown

RNA expression of PVT1 gene in Human Lymphoma tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

RNA expression of PVT1 gene in Human Lymphoma tissue using RNAscope® 2.5 HD Assay Brown

RNA expression of SCHLAP1 gene in human prostate cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

RNA expression of SCHLAP1 gene in human prostate cancer tissue using RNAscope® 2.5 HD Assay Brown

Expression of H19 in Human ovarian cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

Expression of H19 in Human ovarian cancer tissue using RNAscope® 2.5 HD Assay Brown

Expression of MALAT1 in Human Liver cancer tissue using RNAscope<sup>®</sup> 2.5 HD Assay Brown RNA in situ hybridization (ISH)

Expression of MALAT1 in Human Liver cancer tissue using RNAscope® 2.5 HD Assay Brown

Expression of HOTAIR RNA (brown dots) in human breast cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.0 HD Reagent Kit-BROWN

Expression of HOTAIR RNA (brown dots) in human breast cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.0 HD Reagent Kit-BROWN

lncRNA expression of PCA3 RNA (brown dots) in human prostate cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope<sup>®</sup> 2.0 HD Reagent Kit-BROWN

Expression of PCA3 RNA (brown dots) in human prostate cancer FFPE tissue, RNA in situ hybridization (ISH) using RNAscope® 2.0 HD Reagent Kit-BROWN

In the most recent statistics from the GENCODE project (v25, August 2015), the human genome contains 25,823 non-coding RNA (ncRNA) genes, surpassing the number of protein-coding genes (19,815). Of the non-coding RNA species, some 30% (9,882) are less than 200 bases long, termed as small non-coding RNA. They comprise of transfer RNA (tRNA) and ribosomal RNA (rRNA), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNA. About 60% (15,941) of the non-coding RNAs are longer than 200 bases, and are operationally designated as long non-coding RNAs (lncRNAs). The functions of lncRNAs are still being characterized; their abundance and diversity add to the challenge. Some lncRNAs have been shown to regulate gene expression through a diversity of mechanisms and play important roles in chromatin modification (HOTAIR), transcriptional and post-transcriptional regulation (ZEB2). Dysregulation of lncRNA is being found to have relevance not only in tumorigenesis, but also to neurological, cardiovascular, developmental and other diseases.

The discovery of a previously unknown universe of lncRNAs has created an unprecedented demand for effective RNA in situ hybridization tools. Unlike protein coding genes, for which immunohistochemistry (IHC) and RNA in situ hybridization are complementary for mapping gene expression to specific cells in situ, lncRNA expression can only be interrogated by RNA in situ hybridization. The generally lower expression levels of lncRNAs than their protein coding counterparts demand the highest sensitivity from RNA in situ hybridization methods. The single-molecule sensitivity and rapid assay development time (<2 weeks) of ACD’s RNAscope® technology make RNAscope® ideally suited for localizing lncRNAs expression to specific cell types and sub-cellular structures. RNAscope® in situ assays will undoubtedly accelerate lncRNA research and become an indispensable tool for lncRNA-based molecular diagnostics.

Some of the available RNAscope probes for lncRNA - Don’t see your gene of interest? RNAscope® Made-to-Order Target Probes and manufactured within 2 weeks for virtually ANY gene from ANY species in ANY tissue. Learn more at www.acdbio.com/target-probes-made-order








0610012G03RikBREA2EPORLHFPL3-AS2LncI22MYCNOSRAD51-AS1
0610038B21RikC230091D08RikFendrrLINC00152LOC100132735NAALADL2-AS3RoR
1500009C09RikCASC9FLJ32255LINC00162LOC100506013NBR2RP11-255G12-lincRNA
1700019B21RikCCAT1G530011O06RikLINC00302LOC100507412NCBP2-AS2RP23-80I4.3
3110099E03RikCDKN2B-AS1GAS5LINC00473LOC100507506NDRG4SCHLAP1
4833428L15Rikcharon-lncGDNF-AS1-LongNcTvLINC00515LOC100507639Neat1SNHG12
4930463O16RikCHN1Gm10125LINC00538LOC102636514NR_015410SNHG19
6430562O15RikCLEC4GP1Gm15850-LncRNALINC00589LOC157273NronSnhg7
9130024F11RikCLU-tv3Gm2694LINC00704LOC339535Olfr1372spaw-lnc
A330023F24RikCPS1-IT1Gm5083LINC00707LOC400043P2RX7TINCR
A930006I01RikCRNDEGpr19LINC00839LUCAT1PCA3TRERNA1
A930011O12RikCTA-204B4.6H19LINC00844MALAT1PCAT1Ttc39aos1
ADAMTS9-AS2CYP3A5HCP5LINC00887MEG3PCAT14TUG1
AJAXC1-ncRNADANCRHmga2-ps1LINC00941Meg3-tv3PCGEM1UCA1
AOC4PDBETHOTAIRLINC01133MESTIT1PRNCR1UNK-ncRNA
ApelaDISC2HOXB-AS51LINC01212MhrtNR_030682WNT2
ATXN3DLEU1HOXC-AS3LINC01296MIATPTENP1XIST
BCAR4DUX4L-X-familyIL15LINC01546MIR155HGPVT1Zeb2os
BCYRN1E030019B13RikKCNQ1OT1Linc-KLF14-1MIR503PXN-AS1 
BIN3-IT1EGFR-AS1KDM4A-AS1lnc-FAM164A-1MNX1-AS1RAD21-AS1 

 

A list with all lncRNA publications can be found here

Publications

Long non-coding RNA chromogenic in situ hybridisation signal pattern correlation with breast tumour pathology

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