Seminar Max Planck Institute for Infection Biology, Berlin

The RNAscope® technology is being routinely used for tissue-based gene expression analysis. Unique to this technology, RNAscope® delivers quantitative, sensitive and specific molecular detection of RNA species on a cell-by-cell basis with morphological context in a single assay. This enables researchers to visualize which genes are expressed, localize where they are expressed, and quantify the level of expression. This technology has advanced researchers over the last years and enabled 800 peer-reviewed publications.

Dr. Rajendra Kumar Gurumurthy will start the seminar sharing results and experiences with the RNAscope® technology


In addition, our Senior Director of Scientific Affairs, Dr Emily Park will give an overview on the application of RNAscope in the field of Infectious Disease:

 

Viral Pathogen Detection & Visualization in Tissues: Applications of RNAscope® and BaseScope™ ISH technology

Direct detection of viral RNA in human or other animal cells by RNA in situ hybridization (ISH) is a powerful tool to establish the etiology and pathogenesis of viral diseases. Nucleic acid-based molecular detection methods have revolutionized viral detection, offering several essential advantages such as sensitivity, specificity and speed.  Beyond those stated advantages, RNAscope®  ISH uniquely offers molecular detection coupled with morphological context enabling visualization of the virus in different infected tissues and cell types. 


Key features
 

  • Sensitivity—with single RNA molecule detection technology, RNAscope® ISH routinely identify individual viral particles in infected cells despite low or undetectable viral loads. Its high sensitivity level enables detection even in early stages of infection.
  • Specificity—proprietary probe design strategy enables accurate detection among highly related viral species/strains, providing accurate detection even in the presence of contaminants or similar/related viruses.
  • Localization—providing both accurate molecular detection and visualization of infected cell types and tissues enables elucidation of viral transmission, pathogen-host interactions, localization of hidden reservoirs, etc.
  • Coinfection detection—RNAscope® duplex and multiplex assays offer the possibility of detecting multiple viruses or co-infection.
  • Differential detection—flexible probe design targeting the sense or anti-sense strand enables detection and differentiation of virus during latent or active stages
Location: Max Planck Institute for Infection Biology Berlin, Germany, Seminar room

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