RNAscope® 2.5 VS Reagent Kit – RED

The RNAscope® 2.5 VS Assay is designed as an automated assay on the Discovery Ultra and Discovery XT Automated IHC/ISH slide staining systems from Roche. The RNAscope® 2.5 VS Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH.

The RNAscope® 2.5 VS Reagent Kit-RED with it Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed,  ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope. 

These new RNAscope® 2.5 VS reagents required the new RNAscope® 2.5 VS Probes for optimal performance. Probe designs are identical to previous version, however the probes are manufactured for optimized performance with RNAscope® 2.5 reagent chemistry.

Sample Images
RNAscope<sup>®</sup> 2.5 VS Red - Human Tonsil - PPIB

RNAscope® 2.5 VS Red - Human Tonsil - PPIB

RNAscope<sup>®</sup> 2.5 VS Red - Human Ovary - PPIB

RNAscope® 2.5 VS Red - Human Ovary - PPIB

RNAscope<sup>®</sup> 2.5 VS Red - Human Lung - PPIB

RNAscope® 2.5 VS Red - Human Lung - PPIB

RNAscope<sup>®</sup> 2.5 VS Red - Human Esophogus - PPIB

RNAscope® 2.5 VS Red - Human Esophogus - PPIB

The RNAscope® 2.5 VS Automated Workflow requires the following items from Ventana Medical System  (ventanadiscovery.com):

CAT #Product Name and Description
760-224mRNA DAB Detection Kit
760-223mRNA Pretreatment Kit
760-222mRNA Probe Amplification Kit
760-225(one part number that will deliver all three kits 224, 223, 222)
960-785User Fillable Probe Dispenser


RNASCOPE® 2.5 VS Automated Assay—RED Workflow requires the following items from ACD:

  • RNAscope®  2.5 VS Target Probes (Catalog or Made-to-Order LS Probes)
  • RNAscope®  2.5 VS Control Probes (Select species-specific LS positive control probes or packs of probes and LS negative control probes)
  • RNAscope® Control Slides (optional)
  • RNAscope® 2.5 VS  Reagent Kit—RED

Product Retirement:

Effective June 30th 2016, RNAscope 2.0 product line will be discontinued and no longer available as a catalog product. This includes the following: RNAscope 2.0 Brown (Cat No 310033 or 310035), RNAscope 2.0 Red (Cat No 310036 or 310034),RNAscope 2.0 LS Brown (Cat No 321100), RNAscope 2.0 LS Red (Cat No 321130), RNAscope 2.0 VS Brown (Cat No 320600),RNAscope 2.0 VS Red (Cat No 320610).

Please refer to the FAQ for more information about 2.0 discontinuance.

More information on RNAscope 2.5 can be viewed in the Webinar titled “RNAscope® New Product Development- learn more about RNAscope 2.5 product line” here

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