Getting Started and Troubleshooting Tips for RNAscope® and BaseScope™ Assays
Marie Lauigan
Technical Support Scientist
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Outline:

The RNAscope® and BaseScope™ Assays are based on the proprietary RNAscope® Technology, enabling RNA target expression analysis within intact cells and tissues with high sensitivity and specificity. These represent major advancements in RNA ISH approaches.

 

Main features of this technology are:

  • Proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridization
  • Capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments
  • Advances to this technology enabling detection of any mRNA, short RNA targets e.g. transcript variants and exon junctions

 

RNAscope® and BaseScope™ Assays are ideal tools to complement technologies like IHC, qPCR or NGS. In this webinar we will discuss the benefits of both RNAscope® and BaseScope™ assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results. We will also highlight some examples of data for stem cell research specific applications, such as analysis of LRG5 expression.

 

Who should attend:

This webinar will benefit both new and experienced RNAscope® technology users.

Learning Objectives:

How RNAscope® and BaseScope™ assays work

What tools are needed for getting started

Tips and tricks for sample preparation

Examples of staining results

FAQs & Troubleshooting tips

Where to find information

Examples of data featuring research applications

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