Elucidation of stem cell dynamics via Rspondin3 regulation using multiplex RNA in situ hybridization techniques
Dr Michael Sigal
Group Leader, Department of Internal Medicine, Charite-Universitatsmedizin Berlin
Watch Video:
Dr Courtney Anderson
Applications Leader, Senior Scientist, Advanced Cell Diagnostics
Outline:

The stomach epithelium is capable of constant regeneration driven by long-lived resident stem cells, but the mechanism that regulates their identity and turnover is not well known.



In this webcast, the speakers will demonstrate that Axin2+/Lgr5- cells constitute a highly proliferative, non-differentiated compartment capable of regenerating entire antral glands and that Rspo3 is a critical niche-specific regulator that orchestrates stem cell homeostasis. The ability of stromal niche cells (Rspo3+) to control and adapt the dynamics of epithelial stem cells (Axin2+/Lgr5-) constitutes a sophisticated mechanism that orchestrates epithelial regeneration and maintenance of tissue integrity.



During this webcast you will learn how the RNAscope® Dual ISH technology was utilized to thoroughly characterize the gastric antral gland stem cell niche. 

 

Watch the On-Demand Recording here

Learning Objectives:
  • R-spondin family members enhance Wnt signaling
  • Of the 4 Rspo members examined, only Rspo3 was expressed in the mouse antrum, specifically in the stroma adjacent to the stem cell compartment
  • Dual RNA ISH showed that Rspo3 is produced by alpha-smooth muscle actin (α-SMA)-positive myofibroblasts in lamina muscularis mucosa directly beneath the glands
  • The increase in stem cell turnover in response to the gastric pathogen H. pylori is driven by Rspo/Wnt signaling
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