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Advanced Cell Diagnostics’ RNAscope^® platform is fast becoming a routinely used method for target validation and biomarker development based on in situ gene expression analysis. ACD has over 9000 target RNA-specific probes in use and there are over 850 publications citing this technology. RNAscope^® technology is enabling faster and more rigorous target and biomarker development: it is a robust and ultra-sensitive RNA in situ hybridization (RNA ISH) method providing single-cell and single-molecule RNA detection in the context of complex tissue microenvironments. In this webinar the RNAscope^® technology and published applications will be presented by ACD. Quantitative analysis of single/dual chromogenic probes and multiplex fluorescent probes using the HALO platform will be demonstrated by Indica Labs.