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A Cellular and Molecular Phenotyping Pipeline for Target Characterization in FFPE Biobank Samples
Dr. Kjetil Ask
Associate Professor in the Department of Medicine, McMaster University
Megan Vierhout
Masters Candidate, McMaster University
Farzaneh Tondnevis - Ph.D.
Senior Automation Scientist, Advanced Cell Diagnostics
Outline:

This webinar demonstrates how a research team at the Firestone Institute for Respiratory Health at McMaster University developed a cellular and molecular phenotyping pipeline using archived samples of lung tissue derived from patients diagnosed with fibrotic interstitial lung disease. Dr. Kjetil Ask discusses how the clinically relevant tissues were obtained, clinically categorized and deidentified. He provides his perspective on the requirement of cross-disciplinary expertise and the associated technological platform required for the identification and quantification of cellular and molecular targets in fibrotic lung disease. Dr. Ask also discusses the potential application of this platform to pre-clinical models of lung disease. Megan Vierhout presents her research on the role of alternatively activated macrophages and endoplasmic reticulum stress in fibrotic lung disease. She shows specific examples of automated immunohistochemistry and associated in situ hybridization technology using ACD RNAScope and BaseScope assays to demonstrate molecular phenotyping and target identification. Watch the On-Demand Version Here

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