Tips, Tricks & Troubleshooting to Get Publication Quality RNA ISH Data
Nidhi Vashistha, Ph.D
Technical Support Scientist
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Outline:

RNAscope® Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. It represents a major advancement in RNA ISH approaches.

Main features of this technology are:

  • Proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridization
  • Capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments
  • Advances to this technology enabling detection of any mRNA, short RNA targets and exon junctions.

It is an ideal tool to complement technologies like IHC, qPCR or NGS. In this webinar we will discuss the benefits of both RNAscope® and BaseScope™ assays and share tips and tricks on how to perform these assays successfully to obtain publication-quality RNA in situ results. We will also highlight some examples of data for stem cell research specific applications, such as analysis of LRG5 expression.

 

 

Who should attend:

This webinar will benefit both new and experienced RNAscope® technology users.

Learning Objectives:
  • How RNAscope® and BaseScope™ assays work
  • What tools are needed for getting started
  • Tips and tricks for sample preparation
  • FAQs & Troubleshooting tips
  • Where to find information
  • Examples of data featuring stem cell research application
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