RNAscope® 2.5 HD Reagent Kit-RED
Catalog No. 322350
OR

RNAscope® 2.5 HD Reagent Kit-RED

Overview

Description

The RNAscope® 2.5 High Definition(HD)- RED Assay, is based on ACD's patented signal amplification and background suppression technology. The 2.5 HD version is a high sensitive RNA ISH technique and can be used for low expressing gene targets. The RNAscope® 2.5 HD Reagent Kit-RED assay includes the Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope.Each kit contains three sub-kits: RNAscope® 2.5 HD Detection Kit (RED) (Cat# 322360), RNAscope® 2.5 Pretreat Reagents-H202 and Protease Plus (Cat#322330), RNAscope® Target Retrieval (Cat#322000), RNAscope® Wash Buffer (Cat#310091).Disclaimer: Photos for 2.5 products will be updated shortly.
Sample Type Compatibility
Adherent cells, Cell pellets, FFPE, Fixed frozen tissue, Fresh frozen tissue, TMA

RNAScope Workflow

  • Permeabilize
  • Hybridize
  • Amplify
  • Visualize

Specifications

Shipping temperature(°C): 4 ᄚC
Storage temperature(°C): 4 ᄚC
Shelf life: 9 months from the date of manufacturing.
  • * Please check expiration dates on the reagent package
Product Documents

Product Documents

Frequently used with this product

Frequently used with this product

ACD EZ-Batch Slide System
BioCare EcoMount (100mL)
ImmEdge Hydrophobic Barrier Pen
RNAscope® Negative Control Probe - DapB
RNAscope® Positive Control Probe - Hs-POLR2A
RNAscope® Positive Control Probe - Hs-PPIB