RNAscope® 2.5 HD Duplex Reagent Kit
Catalog No. 322430

RNAscope® 2.5 HD Duplex Reagent Kit



RNAscope® 2.5 HD Duplex Reagent Kit, is a RNA in situ hybridization (ISH) chromogenic assay designed for multiplexing up to two targets. It uses HRP (Green) and Fast Red chromogenic stains and the probe names often include C1 or C2. It is ideal for simultaneous detection of two RNA species using RNA ISH. Each RNAscope® Duplex Reagent Kit provides enough reagents to stain ~20 tissue sections, each with an area of approximately 20 mm x 20 mm (0.75in x 0.75in). Each kit contains: RNAscope® Duplex Detection Kit (PN 322500) RNAscope® Pretreatment Reagents (PN 322000, 322330), RNAscope® Wash Buffer (PN 310091).
Sample Type Compatibility
Adherent cells, Cell pellets, FFPE, Fixed frozen tissue, Freshfrozen tissue, TMA

RNAScope Workflow

  • Permeabilize
  • Hybridize
  • Amplify
  • Visualize


Shipping temperature(°C): 4 ᄚC
Storage temperature(°C): 2-8 C
Shelf life: 9 months from the date of manufacturing.
  • * Please check expiration dates on the reagent package
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