RNAscope ISH

Our RNAscope methodology ensures all due care is taken with your samples, and that the assay development and analysis are conducted as efficiently as possible.

Tissue Processing

Upon receipt of the study samples, a qualified histotechnician sections the samples as per ACD’s recommended guidelines to minimize turnaround time and to ensure freshness.

RNAscope® Probe Verification Services

ACD provides custom probe design services using the proprietary ACD RNAscope® probe design pipeline for any proprietary or novel gene target of interest. Custom target probes can be developed within 2 weeks. Catalog probes are available off-the-shelf and can be used right away in Pharma Assay Services.  Probe validation services can be performed on tissues or cell pellet samples.

Tissue Quality Control & Assay Optimization

Pretreatment conditions to permeabilize samples are highly dependent on the tissue type and quality. A tissue quality control step is performed using a positive control gene (PPIB) and negative control gene (bacterial dapB).  This step is critical in determining the tissue and RNA quality of the sample to avoid false positives and false negatives. It is also essential in establishing the optimal pretreatment conditions for your samples to maximize signal to noise.

Optimization is performed by our team of experts, to ensure that you are receiving the best possible data from your samples and these optimized conditions can be transferred directly to your lab. These QC and optimization steps are performed in every Pharma Assay Service project and guarantee the best RNA ISH sensitivity and dynamic range for your specific target(s).

Target Analysis

The optimized pretreatment conditions established during assay optimization are used to perform your target evaluation. Staining can be performed using the manual assay or using the Leica Bond or Ventana Discovery XT or Ultra platforms for automated analysis. Once optimized conditions have been established, the addition of multiple targets requires no additional optimization  

Semi-Quantitative and Quantitative Analysis

We offer 2 levels of analysis to address your specific needs: semi-quantitative and quantitative analysis using SpotStudio or HALO software.  All analyses are performed by our team of qualified experts.

Learn more about these different options.

Summary Report

A complete summary report is an option for every project. It includes optimized protocol and assay conditions, QC results, representative images of control and target staining, materials and methods, and scoring and analysis results. 

Download an example of a Summary Report.

All stained slides and remaining unstained slides/tissue blocks are returned to the customer at the conclusion of the project.

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