Four flexible solutions for all your research needs
Independent of your genes of interest, our easy RNA in situ hybridization assay uses common reagents and protocols; providing universal assay conditions. All RNA-ISH kits contain reagents in a convenient ready-to-use (RTU) format, and chromogenic dyes are available to interrogate a single or two genes simultaneously. Most versatile are our RNAscope® Multiplex Fluorescent Reagent Kits, enabling you to interrogate 1, 2 or 3 genes simultaneously.
Automated RNAscope® ISH assays are also available for Leica Systems (LS) and Ventana Systems (VS).
RNAscope® Manual Solutions Brochure
RNAscope® In Situ Hybridization Assay Workflow
Tissue sections or cells are fixed onto slides and pretreated with RNAscope® Pretreatment Kit to unmask target RNA and permeabilize cells.
Designed with ~20 target-specific double Z probes, RNAscope® Probes hybridize to target RNA molecules.
RNAscope® Detection Reagents amplify the hybridization signals via sequential hybridization of amplifiers and label probes.
Each punctate dot signal represents a single test target RNA molecule and can be visualized with a microscope.
Single-molecule signals can be quantified on a cell-by-cell basis by manual counting or automated image analysis using HALO Software or RNAscope® SpotStudio™ Software.