Best-in-class RNA ISH for your automated systems of choice
For labs that require high throughput and automated RNA in situ hybridization (ISH) analysis, ACD has partnered with Leica Biosystems and Ventana Medical Systems, Inc to deliver RNAscope ISH automated assays.
RNAscope® 2.5 LS Reagent Kits and RNAscope® LS Duplex Reagent Kits are offered for a fully automated walk-away solution on the Leica BOND RX System by Leica Biosystems automating the Permeabilize, Hybridize and Amplify steps of the RNAscope® Workflow.
RNAscope® 2.5 VS Reagent Kits are offered for an automated walk-away solution on the DISCOVERY ULTRA and DISCOVERY XT automated tissue staining systems by Ventana Medical Systems, Inc automating the Hybridize and Amplify steps of the RNAscope® Workflow.
RNAscope® Automated LS Assay
RNAscope® Automated VS Assay
RNAscope® In Situ Hybridization Assay Workflow
Tissue sections or cells are fixed onto slides and pretreated with RNAscope® Pretreatment Kit to unmask target RNA and permeabilize cells.
Designed with ~20 target-specific double Z probes, RNAscope® Probes hybridize to target RNA molecules.
RNAscope® Detection Reagents amplify the hybridization signals via sequential hybridization of amplifiers and label probes.
Each punctate dot signal represents a single test target RNA molecule and can be visualized with a microscope.
Single-molecule signals can be quantified on a cell-by-cell basis by manual counting or automated image analysis using HALO Software or RNAscope® SpotStudio™ Software.