RNAscope® 2.5 VS Reagent Kit – BROWN

The RNAscope® 2.5 VS Assay is designed as an automated assay on the Discovery Ultra and Discovery XT Automated IHC/ISH slide staining systems from Roche. The RNAscope® 2.5 VS Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH.

RNAscope® 2.5 VS Reagent Kit—BROWN is a very robust assay gives reliable results which are ideal for archiving due to the permanent staining. It is the standard in molecular pathology diagnostics, usable for a wide source of samples and the results are visible under any standard bright field microscope. The RNAscope® 2.5 VS Reagent Kit—BROWN  is designed to provide more intense Diaminobenzidine (DAB) staining—ideal when low copy target gene expression is anticipated (1–20 copies per cell). The stained slides are visualized with brightfield microscopes. 

These new RNAscope® 2.5 VS reagents required the new RNAscope® 2.5 VS Probes for optimal performance. Probe designs are identical to previous version, however the probes are manufactured for optimized performance with RNAscope® 2.5 reagent chemistry.

Sample Images
RNAscope<sup>®</sup> 2.5 Brown - Human Skin - PPIB

RNAscope® 2.5 Brown - Human Skin - PPIB

RNAscope<sup>®</sup> 2.5 VS Brown - Human Skin - PPIB

RNAscope® 2.5 VS Brown - Human Skin - PPIB

RNAscope<sup>®</sup> 2.5 VS Brown - Human Cervix - PPIB

RNAscope® 2.5 VS Brown - Human Cervix - PPIB

RNAscope<sup>®</sup> 2.5 VS Brown - Human Esophogus - POLR2A

RNAscope® 2.5 VS Brown - Human Esophogus - POLR2A

The RNAscope® VS Automated Assay workflow requires the following from Ventana Medical Systems (ventanadiscovery.com):

CAT #Product Name and Description
760-224mRNA DAB Detection Kit
760-223mRNA Pretreatment Kit
760-222mRNA Probe Amplification Kit
760-225(one part number that will deliver all three kits 224, 223, 222)
960-785User Fillable Probe Dispenser


RNAscope® VS Automated Assay—BROWN Workflow requires the following items from ACD:

  • RNAscope® 2.5 VS Target Probes (Catalog or Made-to-Order VS Probes)
  • RNAscope® 2.5 VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
  • RNAscope® Control Slides (optional)
  • RNAscope® 2.5 VS Assay Reagent Kit—BROWN 

Product Retirement:

Effective June 30th 2016, RNAscope 2.0 product line will be discontinued and no longer available as a catalog product. This includes the following: RNAscope 2.0 Brown (Cat No 310033 or 310035), RNAscope 2.0 Red (Cat No 310036 or 310034),RNAscope 2.0 LS Brown (Cat No 321100), RNAscope 2.0 LS Red (Cat No 321130), RNAscope 2.0 VS Brown (Cat No 320600),RNAscope 2.0 VS Red (Cat No 320610).

Please refer to the FAQ for more information about 2.0 discontinuance.

More information on RNAscope 2.5 can be viewed in the Webinar titled “RNAscope® New Product Development- learn more about RNAscope 2.5 product line” here

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