RNAscope® 2.5 LSx Reagent Kit-BROWN

Obtain exceptional sensitivity with RNAscope ® 2.5 LS Assay - now even faster with a simpler run set-up. Advanced Cell Diagnostics introduces RNAscope®2.5 LSx Reagent Kit - BROWN, our first pre-filled assay designed to be used as an automated assay on the Leica Biosystems' BOND RX Research Advanced Staining System.  With volumes to accommodate the processing of 60 slides, the RNA ISH reagents come pre-filled in Bond Rx containers fixed on two trays and include DAB chromogen, hematoxylin, bluing and protease.   

The RNAscope®2.5 LSx Reagent Kit - BROWN is a robust assay that produces reliable results, ideal for archiving due to permanent staining. This kit utilizes Diaminobenzidine (DAB) dye, the standard in molecular pathology diagnostics and is ideal when low copy target gene expression is anticipated (1-20 copies per cell).  The results are visible under standard bright field microscopy and are comparable to the existing RNAscope ® 2.5 LS assay.

Sample Images
RNAscope<sup>®</sup> 2.5 LSx Reagent Kit-BROWN Pre-filled Trays

RNAscope® 2.5 LSx Reagent Kit-BROWN Pre-filled Trays

Probe Compatibility

The RNAscope® 2.5 LSx reagents utilize the existing RNAscope® 2.5 LS Probes for optimal performance.

 

Simple and Rapid Run Set Up

The RNAscope®  2.5 LSx Reagent Kit- BROWN with pre-assembled reagent trays significantly decreases the set up time at the bench and instrument. Furthermore, the barcoded containers enable accurate scanning and recording of reagents lot numbers. Run preparation is simple:

  1. Scan and register trays and reagents
  2. Establish run parameters
  3. Select staining protocol
  4. Start your run

Product Configuration

ReagentTray PositionQuantity
RNAscope® 2.5 LSx RinseTray 1, position 1,227 mL x 2 containers
RNAscope® 2.5 LSx HematoxylinTray 1, position 39 mL x 1 container
RNAscope® 2.5 LSx DAB Part 1Tray 1, position 41.2 mL x 1 container
RNAscope® 2.5 LSx DAB Part BTray 1, position 522 mL x 1 container
RNAscope® 2.5 LSx BluingTray 1, position 69 mL x 1 container
RNAscope® 2.5 LSx AMP 1 DABTray 2, position 118 mL x 1 container
RNAscope® 2.5 LSx AMP 2 DABTray 2, position 218 mL x 1 container
RNAscope® 2.5 LSx AMP 3 DABTray 2, position 318 mL x 1 container
RNAscope® 2.5 LSx AMP 4 DABTray 2, position 418 mL x 1 container
RNAscope® 2.5 LSx AMP 5 DABTray 2, position 518 mL x 1 container
RNAscope® 2.5 LSx AMP 6 DABTray 2, position 618 mL x 1 container
RNAscope® 2.5 LSx H2O2Tray 2, position 79 mL x 1 container
RNAscope® 2.5 LSx ProteaseTray 2, position 812 mL x 1 container
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