Immuno-Oncology Datasets

RNAscope® in situ hybridization target datasets provide highly specific and sensitive expression data to complement or substitute for immunohistochemistry studies. RNAscope datasets include many duplexed immuno-oncology and checkpoint target pairs that characterize target relationships, interactions and co-expression patters in tumor microenvironments (TME) of untreated non-small cell lung cancer (NSCLC) tissues.  RNAscope-based target expression analysis is the combination of visualization data and cell-specific molecular quantification in complex TME.

Scientists from ACD’s Pharma Assay Services generated these Immuno-Oncology datasets from RNAscope 2.5 or Duplex assays performed on large (3 mm) core tissue microarrays (TMAs) of tumor and adjacent normal tissues from lung and ovarian cancer patients.

  • High resolution tumor profiling datasets of multiple immune checkpoint and functional markers
  • Readily available cost-effective target expression data
  • Detailed report with high quality images, 40X Aperio full slide scans, and HALO™ quantitative tumor/stroma expression analyses available
Co-expression of PD-L1 and IDO1 in lung cancer: Single-cell analysis by the RNAscope 2.5 HD Duplex Assay revealed co-expression of IDO1(Hs-IDO1; red) with PD-L1(Hs-CD274; green) in a lung cancer tumor core

Co-expression of PD-L1 and IDO1 in lung cancer: Single-cell analysis by the RNAscope 2.5 HD Duplex Assay revealed co-expression of IDO1(Hs-IDO1; red) with PD-L1(Hs-CD274; green) in a lung cancer tumor core

RNAscope® 2.5 HD Duplex and Single-plex Assay Datasets Available:
CCL2/CCR2 CCL2/CCR4 CCL22/CD163 CD44/CD274
CD45/CD274 CD8A/CD274 CD8A/IFNg CD80/CD274
CSF1/CD274 CTLA4** CTLA4/CD274 CTNNB1/CD274
CXCL10/CD68 FOSL1/PD-L1 FOXP3/CD274 GATA3**
HAVCR2/PDCD1 HAVCR2/CD274 ICOS/CD274 IDO1/CD274
IDO1/IFNg IFNg/CD274 IL10/CD274 IL15/CD274
KRT19/CD274 LAG3** LAG3/CD274 LAG3/FOXP3
LAG3/IL3RA LAG3/PDCD1 MET** MET/CD274
MET/EGFR PAX8** PDCD1/CD274 PDCD1LG2/CD274
TGFb1/CD274 TGFb1/COL11A1 TIGIT** TIM3**
TNFRSF9/CD274 TNFRSF9/ICOS TNFRSF18** TNFRSF18/CD274
TNFRSF18/PDCD1 VTCN1/CD274    

* Additional data sets with partial results: 30 tissue cores only: CCL3/CCR1, CCL3/CCR4, CCL3/CCR5.

** Available as single-plex datasets using the RNAscope® 2.5 HD Assay-Brown.

Each NSSCLC dataset, based on assays run on 2 TMA slides, includes:

  • High resolution Aperio 40x full slide scans
  • Project Report with representative images showing range of target expression at low and high magnification in tumor and stroma region, material and methods, tabulated control probe QC scores, excel file format annotation of all tissues present on the TMA

HALO image analysis software (Indica Labs) was also used to quantify marker expression. Image analysis data (recommended add-on option) includes:

  • Quantification of percentage of cells positive (%+) for each marker in tumor and stroma regions of interest (ROI)
  • Quantification of percentage of cells dual-positive (%+,+) for each marker pair in tumor and stroma ROI
  • H-scores for expression level of each marker in tumor and stroma ROI
  • Representative mark-up images showing ROI on which image analysis quantification was performed

 

Immuno-Oncology Datasets

 

View an example of a comprehensive report that demonstrates:

  • Wide range of PD-L1 & IDO1 co-expression in NSCLC tumor & normal tissues 
  • Intra-tumor expression heterogeneity
  • Primary tumor and stromal expression level

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