Learn more about our New BaseScopeTM assay

Halo Software by Indica Labs

ACD has partnered with Indica Labs to offer quantitative RNAscope image analysis. This advanced digital pathology image analysis solution brings objective and accurate quantification to RNA in situ hybridization, enabling a new generation of applications based on single-cell analysis. With HALO, gene expression can be measured quantitatively at single-cell resolution and interpreted by pathologists within the histopathological context.

Sample Images
HALO exports summary analysis data or cell-by-cell data in csv file format or allows users to generate summary analysis documents as shown above.

HALO exports summary analysis data or cell-by-cell data in csv file format or allows users to generate summary analysis documents as shown above.

HALO 2.0 user interface displaying the results section. The panel to the left includes summary analysis data, histograms, and cell-by-cell data. A cell of interest is highlighted in both the cell table and the image. Enlarged histogram displayed at right.

HALO 2.0 user interface displaying the results section. The panel to the left includes summary analysis data, histograms, and cell-by-cell data. A cell of interest is highlighted in both the cell table and the image. Enlarged histogram displayed at right.

RNA ISH detection with duplex red/green chromogens and hematoxylin counterstain. HALO markup image shows the ISH Module markup image for dual probe detection across the entire slide. Heatmaps indicate the number of probe copies per cell across the tissue.

RNA ISH detection with duplex red/green chromogens and hematoxylin counterstain. HALO markup image shows the ISH Module markup image for dual probe detection across the entire slide. Heatmaps indicate the number of probe copies per cell across the tissue.

FISH detection with red/green probes and DAPI counterstain. Markup images show HALO-FISH Module markup images for multi and single probe detection. Nucleus color represents the cell spot count. Darker shades indicate higher spot counts.

FISH detection with red/green probes and DAPI counterstain. Markup images show HALO-FISH Module markup images for multi and single probe detection. Nucleus color represents the cell spot count. Darker shades indicate higher spot counts.

RNA ISH detection with duplex red/brown chromogens and hematoxylin counterstain. Markup images show HALO-ISH Module markup images for dual and single probe detection. Nucleus color represents the cell spot count. Darker shades indicate higher spot counts.

RNA ISH detection with duplex red/brown chromogens and hematoxylin counterstain. Markup images show HALO-ISH Module markup images for dual and single probe detection. Nucleus color represents the cell spot count. Darker shades indicate higher spot counts.

The benefits of image analysis using HALO software after RNAscope staining include:

  • quantitative and reproducible RNAscope spot counting
  • single-plex and multiplex ISH quantification
  • brightfield and fluorescent ISH quantification
  • cell-by-cell expression profiles
  • high throughput computing power for faster turnaround time
  • whole-slide image or field-of-view image analysis
  • manual and automatic selection of distinct cell populations

HALO image analysis is compatible with all RNAscope Manual and Automated Assays:

  • RNAscope® 2.0 HD Reagent Kit—BROWN
  • RNAscope® 2.0 HD Reagent Kit—RED
  • RNAscope® 2-Plex Reagent Kit
  • RNAscope® Multiplex Fluorescent Reagent Kit
  • RNAscope® LS Reagent Kit—BROWN
  • RNAscope® LS Reagent Kit—RED
  • RNAscope® VS Reagent Kit—BROWN
  • RNAscope® VS Reagent Kit—RED

ACD offers image analysis as part of our Pharma Assay Services (Learn more)

The HALO image analysis software is also available for purchase and deployment within your organization. For more information contact info@indicalab.com or visit Indicalab.com.

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