Documents and Downloads

Application Notes and Reviews

Cancer

Application Review

Here we summarize the role of novel RNA in situ hybridization technology in expanding the tool-set available to researchers, thereby overcoming the challenges of cancer research in four key areas:

  1. Analysis of gene expression to understand tumor heterogeneity
  2. Studying non-coding RNAs
  3. Developing biomarkers
  4. Driving and refining the diagnostic strategies of the future

Visualizing the Cancer Transcriptome - How RNA in situ Hybridization Offers an Essential Data Dimension in Cancer Research

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Neuroscience

Application review

Learn more in our RNAscope® Neuroscience Application Note - Detection of RNA in the central and peripheral nervous system using the RNAscope® in situ hybridization assay

Detection of RNA in the central and peripheral nervous system using the RNAscope® in situ hybridization assay

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Stem Cell

Application Review

Cellular localization of RNA Expression in Stem Cells using RNAscope® Technology

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Inflammation

Application review

Specific Detection of Inflammation Markers within Tissue Context Using RNAscope® Technology

Specific Detection of Inflammation Markers with Tissue Context Using RNAscope® Technology

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HPV RNA Detection

RNAscope® HPV Application Review

Read more about how to asses transcriptionally active HPV biomarkers in our RNAscope® HPV Application Review.

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Preclinical safety assessment

Drug Safety Assessment Poster Presentation

 

STP Poster 2015
"Fully Automated RNA in situ Hybridization for Proliferation and Apoptosis-related Biomarkers in Preclinical Animal Model Tissue Panels using RNAscope® 2.5 LS Assay"

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Application Note

Application Note "Robust detection of RNA biomarkers for drug safety assessment in preclinical animal models by fully automated RNAscope® 2.5 LS Assay"

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Appendix

Appendix "Robust detection of RNA biomarkers for drug safety assessment in preclinical animal models by fully automated RNAscope® 2.5 LS Assay"

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Immuno-Oncology

Application note

ACD evaluated the performance of the RNAscope® assay in the detection of a wide range of important immuno-oncology markers, including 12 immune checkpoint markers (i.e., PD-L1, CTLA-4, IDO1), 14 immune cell markers (i.e., CD3, CD4, CD8) and 24 immune function markers (i.e., IFNG,TGFB, IL6, CXCL10) in multiple FFPE human tumor samples.

Application Note

Application note "Detection of immune cell checkpoint and functional markers in the tumor microenvironment by the RNA in situ hybridization RNAscope® assay'

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Appendix

Appendix "Detection of immune cell checkpoint and functional markers in the tumor microenvironment by the RNA in situ hybridization RNAscope® assay"

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Brochures

TitleFile
RNAscope® Automated LS Assay (Leica Brochure) Pdf
RNAscope® Automated VS Assay (Ventana Brochure) Pdf
RNAscope® Manual Solutions Brochure Pdf
RNAscope® Assay Services Brochure Pdf

Probe Lists

TitleFile
RNAscope Cancer Gene List Pdf
RNAscope Long Non-coding RNA Biomarkers Probe List Pdf
RNAscope Stem Cell RNA Biomarkers Probe List Pdf

RNAscope® Catalog Target Probes List (Updated August 2017)

RNAscope® Catalog Target Probes List (Updated August 2017)

Spotlight Interviews

Dr. Patricia Pesavento:Insights into viral pathogenesis.

Interview with Dr. Patricia Pesavento

Insights into viral pathogenesis.

Determination of causation by highly sensitive and specific viral RNA detection and localization with RNAscope® ISH.

“A method as sensitive as RNAscope assay with spatial resolution is absolutely irreplaceable for our confidence in determining viral contribution to a disease.”

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Dr. Ann-Marie Baker:Characterizing LGR5 stem cells in colorectal adenomas and carcinomas.

Interview with Dr. Ann-Marie Baker

Characterizing LGR5 stem cells in colorectal adenomas and carcinomas.

Overcoming the limitations of antibody-based detection methods.

“Using the RNAscope assay probe against LGR5 mRNA has allowed us to study the localization of LGR5 expression without relying on an antibody””

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Dr. Rohit Mehra:Realizing the potential of lon noncoding RNA as a cancer biomarker.

Interview with Dr. Rohit Mehra

Realizing the potential of long noncoding RNA (lncRNA) as a cancer biomarker.

From NGS discovery to validation with RNA in situ Hybridization.

“We found RNAscope technology fast and easy to use, this technology also allows us to directly visualize gene expression in the target tissue of interest ....Also, only one 4 micron FFPE section is sufficient to give us all this information.”

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Dr. Nallasivam Palanisamy:Development of Prostate Cancer Personalized Medicine.

Interview with Dr. Nallasivam Palanisamy

Development of Prostate Cancer Personalized Medicine.

Advancing analysis of prostate tumor molecular heterogeneity by combined immunohistochemistry and novel RNA in situ hybridization.

“Given the unique probe design and detection chemistry of the RNAscope method, we were able to get results with high specificity and sensitivity for many probes that we have used in my laboratory. To date we don’t have any comparable technology for the reliable detection of RNA in FFPE tissues other than RNAscope.”

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Dr. Alexy Pronin:Exploring GPCR expression in the eye.

Interview with Dr. Alexy Pronin

Exploring GPCR expression in the eye.

From discovery with NGS to spatial and quantitative expression confirmation with RNAscope® technology.

“Because RNAscope can detect expression of multiple genes in parallel, it can tell us whether those genes discovered through RT-PCR are co-expressed in the same cell or not – information that is very valuable and hard to obtain by other means.”

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Dr. Sarah Getu and Saskia Smits:Detecting a Novel Circovirus in Foxes with Meningoencephalitis - How custom RNAscope® assay probes are enhancing viral understanding

Interview with Dr. Sarah Getu and Saskia Smits

Detecting a Novel Circovirus in Foxes with Meningoencephalitis - How custom RNAscope® assay probes are enhancing viral understanding

The burden of infectious diseases on human health worldwide has markedly increased over the past few decades, most of these emerging events are caused by viruses originating from the animal world. Understanding the roots of viral behavior in animal hosts is therefore vital and this is a primary focus of Sarah Getu’s and Saskia Smits’s work at the Department of Viroscience, Erasmus MC. In this interview, they explain how the creation of a custom RNAscope® in situ hybridization (ISH) probe allowed them to localize a newly discovered circovirus to neurologic lesions in foxes, providing valuable information to support causation.

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White Paper

Why investigate RNA?

Whitepaper: A guide to RNA as a biomarker and its detection: unlocking the potential of RNA as a clinically valuable biomarker, the latest RNA analysis technologies are changing the way we perceive and utilize this intriguing molecule. In our new free-to-download whitepaper we explore what the RNA Revolution means for biomarkers, now and in the future.

Get it today to learn more about:

The Biomarker: What makes a valuable biomarker, and how a direct path from RNA biomarker discovery to the clinic is vital, avoiding the use of DNA or protein surrogates.

The Method: A biomarker is only as good as its routine analysis methodology, but what constitutes the optimal biomarker method? Advantages and pitfalls of existing methods for routine biomarker analysis will also be discussed.

The Future: How the utilization of RNA as a biomarker is achieved through the latest RNA analysis methods, such as our very own RNAscope®

Whitepaper: A guide to RNA as a biomarker and its detection

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