Advanced Cell Diagnostics’ RNAscope® platform is fast becoming a routinely used method for target validation and biomarker development based on in situ gene expression analysis. ACD has nearly 9000 target RNA-specific probes in use and there are over 400 publications with the method. RNAscope® technology is enabling faster and more rigorous target and biomarker development: it is a robust and ultra-sensitive RNA in situ hybridization (RNA ISH) method providing single-cell and single-molecule RNA detection in the context of complex tissue microenvironments. In this webinar the RNAscope® technology and published applications will be presented by ACD. Quantitative analysis of single/dual chromogenic probes and multiplex fluorescent probes using the HALO platform will be demonstrated by Indica Labs.