Learn more about our New BaseScopeTM assay

Control Probes for Automated Assays: RNAscope® LS Assays

Species-specific positive probes and negative control probes are needed to run with Human or mouse control slides for technique quality control check and with your sample for Sample or RNA quality control check. ACD provides positive control probes that target specie-specific housekeeping genes and negative control probes that target bacteria DapB gene.

Careful selection of the right positive control is recommended.  The expected expression level of your target RNA is the key criteria consider as your positive control probe should have similar expression profile.  See the overview page for details.

 

What to Order

Control probes for RNAscope® 2.5 LS Reagent Kit—BROWN and RNAscope® 2.5 LS Reagent Kit—RED

Control probes for these two assays are interchangeable as both function in Probe Channel 1.  ACD offers over 40 control probes. Use the SEARCH functionality and  enter "RNAscope 2.5 LS Control Probes" and filter for channel 1 probes.

Examples: 

RNAscope® 2.5 LS Negative Control Probe_dapB  Cat. No. 312038

RNAscope® 2.5 LS Positive Control Probe- Hs-UBC Cat No. 312038

RNAscope® 2.5 LS Positive Control Probe_Hs-PPIB Cat No. 313908

 

Control probes for RNAscope® 2.5 LS Duplex Assay:

Product Name & Description

Cat No.

RNAscope® 2.5 LS Duplex Control Probes (PPIB-C1,  Polr2A-C2)- Hu

Pooled Control probes- Control probes in both C1 and C2 channels

320748

RNAscope® 2.5 LS Duplex Control Probes (PPIB-C1,  Polr2A-C2)- Mm 

Pooled Control probes- Control probes in both C1 and C2 channels

320768

RNAscope® 2.5 LS Duplex Negative Control Probe (DapB-C1, DapB-C2)

DapB in both channel 

320758

RNAscope® 2.5 LS Positive Control Probe - Hs-PPIB-C2   

50x Control Probes in C2 channel: These probes can be combined with C1 RTU 

313918-C2

RNAscope® 2.5 LS Negative Control Probe - DapB-C2  

50x Control Probes in C2 channel: These probes can be combined with C1 RTU 

312038-C2

 

Control probes for RNAscope® LS Multiplex Fluorescent Assay:

Product Name & Description

Cat No. 

RNAscope® 2.5 LS 3-Plex Positive Control Probe- Hs

POLR2A (C1 Channel), PPIB (C2 Channel), UBC(C3 Channel)

320868

RNAscope 2.5 LS 3-plex Positive Control Probe--Mn

POLR2A (C1 Channel), PPIB (C2 Channel), UBC(C3 Channel) 

320888

RNAscope® 2.5 LS 3-plex  Positive Control  Probe- HeLa Cells 

TBP (C1 Channel), PPIB (C2 Channel), POLR2A (C3 Channel

320838

RNAscope® 3-plex LS Multiplex Negative Control  Probe 

DapB in all 3 channels 

320878

 

Sample Images
RNAscope<sup>®</sup> 2.5 LS Duplex Assay - mouse brain - Ppib and Polr2a

RNAscope® 2.5 LS Duplex Assay - mouse brain - Ppib and Polr2a

RNAscope<sup>®</sup> 2.5 - Lymph node - dapB

RNAscope® 2.5 - Lymph node - dapB

RNAscope<sup>®</sup> 2.5 - Breast sample - PPIB

RNAscope® 2.5 - Breast sample - PPIB

RNAscope<sup>®</sup> 2.5 - Breast sample - dapB

RNAscope® 2.5 - Breast sample - dapB

X
Contact Us

Complete one of the two forms below and we will get back to you.

Advanced Cell Diagnostics

Our new headquarters office starting May 2016:

7707 Gateway Blvd.  
Newark, CA 94560
Toll Free: 1 (877) 576-3636
Phone: (510) 576-8800
Fax: (510) 576-8798

 

Advanced Cell Diagnostics , S.r.l.

Via Calabria15
20090 Segrate (Milano) - Italy

Phone: +39.02.95360323
Fax: +39.02.95360992
For European orders: order_europe@acdbio.com

Advanced Cell Diagnostics China

Building 15, No. 26 Xihuan South Road
Beijing Economic-Technological Development Area
Beijing, China, Zip code: 100176

400-966-1796
info_china@acdbio.com
For general information: Leon Li lli@acdbio.com
Web: www.acdbio.com.cn

For general information: info@acdbio.com
For place an order: order@acdbio.com
For product support: support@acdbio.com
For career opportunities: hr@acdbio.com