Species-specific positive probes and negative control probes are needed to run with Human or mouse control slides for technique quality control check and with your sample for Sample or RNA quality control check. ACD provides positive control probes that target species-specific housekeeping genes and negative control probes that target bacteria DapB gene.
Careful selection of the right positive control is recommended. The expected expression level of your target RNA is the key criteria consider as your positive control probe should have similar expression profile. See the overview page for details.
When working with RNAscope Multiplex Fluorescent Reagent Kit, you will have ultimate flexibility and can use with one, two or three target RNAs.